Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit | NFYC elisa kit

Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit

Cat. Num. AAA2018862

• Gene Names: NFYC; HSM; CBFC; HAP5; CBF-C; NF-YC; H1TF2A
• Reactivity: Human
• Synonyms: Nuclear Transcription Factor Y Gamma (NFYC); Human Nuclear Transcription Factor Y Gamma (NFYC) ELISA Kit; CBF-C; NFYg; CBFC; H1TF2A; HAP5; HSM; NF-YC; hCBF-C; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; Transactivator HSM-1/2; NFYC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Transcription Factor Y Gamma (NFYC).; No significant cross-reactivity or interference between Nuclear Transcription Factor Y Gamma (NFYC) and analogues was observed.
• Sequence Length: 301

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Transcription Factor Y Gamma (NFYC) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Transcription Factor Y Gamma (NFYC) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFYC elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Transcription Factor Y Gamma (NFYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Transcription Factor Y Gamma (NFYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NFYC elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 217272835
• NCBI GeneID: 4802
• NCBI Accession #: NP_001136062.1
• NCBI GenBank Nucleotide #: NM_001142590.1
• UniProt Accession #: Q13952; Q59GY4; Q5T6K8; Q5T6K9; Q5T6L1; Q5TZR6; Q92869; Q9HBX1; B4DUS6; B4DW63; D3DPV9; F8VWM3
• Molecular Weight: 37,066 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit gamma isoform 5
• NCBI Official Synonym Full Names: nuclear transcription factor Y, gamma
• NCBI Official Symbol: NFYC
• NCBI Official Synonym Symbols: HSM; CBFC; HAP5; CBF-C; NF-YC; H1TF2A
• NCBI Protein Information: nuclear transcription factor Y subunit gamma
• UniProt Protein Name: Nuclear transcription factor Y subunit gamma
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYC
• UniProt Synonym Gene Names: NF-YC
• UniProt Entry Name: NFYC_HUMAN

NCBI Description

This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

NFYC: Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Belongs to the NFYC/HAP5 subunit family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein folding; positive regulation of transcription, DNA-dependent; cellular lipid metabolic process