Mouse anti-Human, Mouse SPRED1 Monoclonal Antibody | anti-SPRED1 antibody

SPRED1 (SPRED1, Sprouty-related, EVH1 domain-containing protein 1) (Biotin)

Cat. Num. AAA6121242

• Gene Names: SPRED1; NFLS; hSpred1; spred-1; PPP1R147
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: SPRED1; Monoclonal Antibody; SPRED1 (SPRED1; Sprouty-related; EVH1 domain-containing protein 1) (Biotin); EVH1 domain-containing protein 1; anti-SPRED1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 462CT2.5.1
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SPRED1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:100-250; Applications are based on unconjugated antibody.
• Immunogen: Purified His-tagged SPRED1 protein (fragment).
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SPRED1 Antibody western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the SPRED1 antibody detected the SPRED1 protein (arrow).)

Related Product Information for anti-SPRED1 antibody

The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). [provided by RefSeq].

Product Categories/Family for anti-SPRED1 antibody

Antibodies; Sprouty

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Batz, C., et al. Blood 115(12):2557-2558(2010) Messiaen, L., et al. JAMA 302(19):2111-2118(2009) Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009) Spurlock, G., et al. J. Med. Genet. 46(7):431-437(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 22749221
• NCBI GeneID: 161742
• NCBI Accession #: NP_689807.1
• NCBI GenBank Nucleotide #: NM_152594.2
• UniProt Accession #: Q7Z699; Q05D53; Q8N256; B2RPJ8
• Molecular Weight: 50,477 Da
• NCBI Official Full Name: sprouty-related, EVH1 domain-containing protein 1
• NCBI Official Synonym Full Names: sprouty related, EVH1 domain containing 1
• NCBI Official Symbol: SPRED1
• NCBI Official Synonym Symbols: NFLS; hSpred1; spred-1; PPP1R147
• NCBI Protein Information: sprouty-related, EVH1 domain-containing protein 1
• UniProt Protein Name: Sprouty-related, EVH1 domain-containing protein 1
• Protein Family: Sprouty-related, EVH1 domain-containing protein
• UniProt Gene Name: SPRED1
• UniProt Synonym Gene Names: Spred-1; hSpred1
• UniProt Entry Name: SPRE1_HUMAN

Uniprot Description

SPRED1: Tyrosine kinase substrate that inhibits growth-factor- mediated activation of MAP kinase. Negatively regulates hematopoiesis of bone marrow. Defects in SPRED1 are the cause of neurofibromatosis type 1-like syndrome (NFLS). It is a disorder characterized mainly by cafe au lait macules without neurofibromas or other tumor manifestations of neurofibromatosis type 1, axillary freckling, and macrocephaly. Additional clinical manifestations include Noonan-like facial dysmorphism, lipomas, learning disabilities and attention deficit-hyperactivity.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane

Molecular Function: phosphatase binding; protein binding; protein kinase binding; protein serine/threonine kinase inhibitor activity; stem cell factor receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; inactivation of MAPK activity; MAPKKK cascade; positive regulation of DNA damage response, signal transduction by p53 class mediator

Disease: Legius Syndrome