Human NRCAM PicoKine ELISA Kit | NRCAM elisa kit

Human NRCAM PicoKine ELISA Kit

Cat. Num. AAA177599

• Reactivity: Human
• Synonyms: NRCAM PicoKine; Human NRCAM PicoKine ELISA Kit; Neuronal cell adhesion molecule; Bravo; hBravo; Neuronal surface protein Bravo; Ng CAM related; Ng-CAM-related; NgCAM related cell adhesion molecule; NgCAM-related cell adhesion molecule; Nr CAM; Nr-CAM; Nrcam; NRCAM_HUMAN ; neuronal cell adhesion molecule; NRCAM elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human NRCAM
• Sequence Length: 1304

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human NRCAM in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, L30-N600
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human NRCAM standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human NRCAM standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|675|1308|2147|694|1382|2503; Standard deviation|35.10|79.78|124.52|40.25|89.83|155.18; CV(%)|5.2|6.1|5.8|5.8|6.5|6.2
• Sample Data: Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000; O.D.|0.058|0.132|0.210|0.338|0.604|1.027|1.531|2.037
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human NRCAM PicoKine ELISA Kit standard curve )

Related Product Information for NRCAM elisa kit

Principle of the assay: Human NRCAM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NRCAM has been precoated onto 96-well plates. Standards (NSO, L30-N600) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NRCAM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NRCAM amount of sample captured in plate.

Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 81158226
• NCBI GeneID: 4897
• NCBI Accession #: NP_001032209.1
• NCBI GenBank Nucleotide #: NM_001037132.2
• UniProt Accession #: Q92823; O15051; O15179; Q14BM2; Q9UHI3; Q9UHI4; A4D0S3; E9PDA4
• Molecular Weight: 131,744 Da
• NCBI Official Full Name: neuronal cell adhesion molecule isoform A
• NCBI Official Synonym Full Names: neuronal cell adhesion molecule
• NCBI Official Symbol: NRCAM
• NCBI Protein Information: neuronal cell adhesion molecule
• UniProt Protein Name: Neuronal cell adhesion molecule
• Protein Family: Neuronal cell adhesion molecule
• UniProt Gene Name: NRCAM
• UniProt Synonym Gene Names: KIAA0343; Ng-CAM-related
• UniProt Entry Name: NRCAM_HUMAN

NCBI Description

Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NRCAM: Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: external side of plasma membrane; extracellular region; integral to plasma membrane; neuron projection; plasma membrane; synapse

Molecular Function: ankyrin binding; protein binding

Biological Process: action potential propagation; angiogenesis; axonal fasciculation; axonogenesis; cell-cell adhesion; central nervous system development; clustering of voltage-gated sodium channels; heterotypic cell-cell adhesion; neuron migration; positive regulation of neuron differentiation; protein localization; regulation of axon extension; retinal ganglion cell axon guidance; synaptogenesis