Human Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) ELISA Kit | NTRK2 elisa kit

Human Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) ELISA Kit

Cat. Num. AAA2025487

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Reactivity: Human
• Synonyms: Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2); Human Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) ELISA Kit; GP145-TrkB; TRK-B; BDNF/NT-3 growth factors receptor; Tropomyosin-related kinase B; TrkB tyrosine kinase; NTRK2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2).; No significant cross-reactivity or interference between Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) and analogues was observed.
• Sequence Length: 822

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NTRK2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NTRK2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 15217077
• NCBI GeneID: 4915
• Molecular Weight: 35,332 Da
• NCBI Official Full Name: neurotrophic tyrosine kinase receptor type 2
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: postsynaptic membrane; growth cone; cell surface; rough endoplasmic reticulum; integral to plasma membrane; presynaptic active zone; dendritic spine; terminal button; excitatory synapse; endosome membrane; perikaryon; cytosol; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; retinal rod cell development; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; regulation of dendrite development; neuron migration; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; aging; central nervous system neuron development; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system neuron development; positive regulation of phosphoinositide 3-kinase cascade; glutamate secretion; long-term memory; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Obesity, Hyperphagia, And Developmental Delay