Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nephronectin (NPNT) Standard Curve.)
Human Nephronectin (NPNT) ELISA Kit | NPNT elisa kit
Human Nephronectin (NPNT) ELISA Kit
Gene Names
NPNT; POEM; EGFL6L
Reactivity
Human
Synonyms
Nephronectin (NPNT); Human Nephronectin (NPNT) ELISA Kit; EGFL6L; POEM; NPNT elisa kit
Reactivity
Human
Specificity
Human Nephronectin (NPNT) ELISA Kit has high sensitivity and excellent specificity for detection of Human NPNT. No significant cross-reactivity or interference between Human NPNT and analogues was observed.
Sequence Length
565
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nephronectin (NPNT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Nephronectin (NPNT) Standard Curve.)
Related Product Information for NPNT elisa kit
Description: This Human Nephronectin (NPNT) ELISA Kit employs a two-site sandwich ELISA to quantitate NPNT in samples. An antibody specific for NPNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPNT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPNT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nephronectin contains 561 amino acids. In situ hybridization detected nephronectin in the developing mouse kidney and in several other tissues and organs. Expression appeared particularly prominent at epithelial-mesenchymal interfaces in tissues undergoing morphogenesis. The deduced 565- and 582-amino acid proteins have calculated molecular masses of 61.9 and 64.0 kD, respectively. Both proteins contain an N-terminal signal sequence, followed by 5 EGF domains, and a C-terminal MAM domain. They also contain an RGD integrin-binding site, a potential glycosylation site, and a potential tyrosine phosphorylation site. EGF repeats 2, 4, and 5 contain Ca(2+)-binding EGF domains. In the longer variant, the 17 additional amino acids are inserted N-terminal to the first EGF domain.
Background: Nephronectin contains 561 amino acids. In situ hybridization detected nephronectin in the developing mouse kidney and in several other tissues and organs. Expression appeared particularly prominent at epithelial-mesenchymal interfaces in tissues undergoing morphogenesis. The deduced 565- and 582-amino acid proteins have calculated molecular masses of 61.9 and 64.0 kD, respectively. Both proteins contain an N-terminal signal sequence, followed by 5 EGF domains, and a C-terminal MAM domain. They also contain an RGD integrin-binding site, a potential glycosylation site, and a potential tyrosine phosphorylation site. EGF repeats 2, 4, and 5 contain Ca(2+)-binding EGF domains. In the longer variant, the 17 additional amino acids are inserted N-terminal to the first EGF domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nephronectin isoform B
NCBI Official Synonym Full Names
nephronectin
NCBI Official Symbol
NPNT
NCBI Official Synonym Symbols
POEM; EGFL6L
NCBI Protein Information
nephronectin
UniProt Protein Name
Nephronectin
Protein Family
UniProt Gene Name
NPNT
UniProt Synonym Gene Names
EGFL6L; POEM
UniProt Entry Name
NPNT_HUMAN
Uniprot Description
NPNT: Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha- 8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: proteinaceous extracellular matrix; smooth muscle contractile fiber; extracellular region; basement membrane
Molecular Function: integrin binding; calcium ion binding
Biological Process: positive regulation of osteoblast differentiation; establishment of protein localization; extracellular matrix organization and biogenesis; ureteric bud development; ureteric bud branching; cell-matrix adhesion; cell-cell adhesion mediated by integrin; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of smooth muscle contraction
Research Articles on NPNT
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Product Notes
The Human NPNT npnt (Catalog #AAA9715879) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715879 ELISA Kit recognizes Human NPNT. It is sometimes possible for the material contained within the vial of "Nephronectin (NPNT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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