Human Nectin-3/CD113 ELISA Kit | CD113 elisa kit
Human Nectin-3/CD113 PicoKine ELISA Kit
Gene Names
NECTIN3; PPR3; PRR3; CD113; PVRL3; PVRR3; CDW113; NECTIN-3
Reactivity
Human
Synonyms
Nectin-3/CD113; Human Nectin-3/CD113 PicoKine ELISA Kit; Human Nectin-3/CD113; CD113 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Nectin-3/CD113
Sequence Length
549
Assay Range
125pg/ml-8000pg/ml
Sensitivity
<20pg/ml
Capture Antibody
A monoclonal antibody from mouse.
Detection Antibody
A biotinylated detection polyclonal antibody from goat.
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml): |159|873|3809|239|939|3980
Standard Deviation: |10.65|45.40|217.11|15.06|50.71|163.18
CV(%): |6.7%|5.2%|5.7%|6.3%|5.4%|4.1%
n|16|16|16|24|24|24
Mean(pg/ml): |159|873|3809|239|939|3980
Standard Deviation: |10.65|45.40|217.11|15.06|50.71|163.18
CV(%): |6.7%|5.2%|5.7%|6.3%|5.4%|4.1%
Sample Data
Concentration (pg/ml): |0|125|250|500|1000|2000|4000|8000
OD: |0.110|0.183|0.230|0.386|0.596|0.991|1.661|2.295
OD: |0.110|0.183|0.230|0.386|0.596|0.991|1.661|2.295
Related Product Information for CD113 elisa kit
Description: For quantitative detection of human Nectin-3/CD113 in cell culture supernates, serum and plasma (heparin, EDTA).
Background: Human Nectin-3/CD113 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-3/CD113 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G58-T404) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-3/CD113 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. It is mapped to 19q13.32. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.
Background: Human Nectin-3/CD113 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-3/CD113 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G58-T404) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-3/CD113 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate. Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. It is mapped to 19q13.32. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nectin-3 isoform 1
NCBI Official Synonym Full Names
nectin cell adhesion molecule 3
NCBI Official Symbol
NECTIN3
NCBI Official Synonym Symbols
PPR3; PRR3; CD113; PVRL3; PVRR3; CDW113; NECTIN-3
NCBI Protein Information
nectin-3
NCBI Description
This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]
Research Articles on CD113
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Product Notes
The Human CD113 (Catalog #AAA1751705) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751705 ELISA Kit recognizes Human CD113. It is sometimes possible for the material contained within the vial of "Nectin-3/CD113, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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