Human MutL Homolog 3 (MLH3) ELISA Kit | MLH3 elisa kit

Human MutL Homolog 3 (MLH3) ELISA Kit

Cat. Num. AAA2025494

• Gene Names: MLH3; HNPCC7
• Reactivity: Human
• Synonyms: MutL Homolog 3 (MLH3); Human MutL Homolog 3 (MLH3) ELISA Kit; HNPCC7; DNA mismatch repair protein Mlh3; MLH3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MutL Homolog 3 (MLH3).; No significant cross-reactivity or interference between MutL Homolog 3 (MLH3) and analogues was observed.
• Sequence Length: 1453

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MutL Homolog 3 (MLH3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MutL Homolog 3 (MLH3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MLH3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MutL Homolog 3 (MLH3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MutL Homolog 3 (MLH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MutL Homolog 3 (MLH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MutL Homolog 3 (MLH3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MLH3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119601618
• NCBI GeneID: 27030
• Molecular Weight: 161,021 Da
• NCBI Official Full Name: mutL homolog 3 (E. coli), isoform CRA_b
• NCBI Official Synonym Full Names: mutL homolog 3
• NCBI Official Symbol: MLH3
• NCBI Official Synonym Symbols: HNPCC7
• NCBI Protein Information: DNA mismatch repair protein Mlh3
• UniProt Protein Name: DNA mismatch repair protein Mlh3
• Protein Family: DNA mismatch repair protein
• UniProt Gene Name: MLH3
• UniProt Entry Name: MLH3_HUMAN

NCBI Description

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MLH3: Probably involved in the repair of mismatches in DNA. Defects in MLH3 are the cause of hereditary non-polyposis colorectal cancer type 7 (HNPCC7). Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Defects in MLH3 are a cause of colorectal cancer (CRC). Belongs to the DNA mismatch repair MutL/HexB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: male germ cell nucleus; chiasma; synaptonemal complex; nucleus

Molecular Function: centromeric DNA binding; protein binding; ATPase activity; satellite DNA binding; chromatin binding; single-stranded DNA binding; ATP binding; mismatched DNA binding

Biological Process: synaptonemal complex assembly; protein localization; mismatch repair; meiotic recombination; male meiosis; female meiosis I

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 7; Endometrial Cancer