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Typical Testing Data/Standard Curve (for reference only)

Human MMP-2 ELISA Kit

MMP-2 (Human) ELISA Kit

Reactivity
Human
Synonyms
MMP-2; MMP-2 (Human) ELISA Kit; Matrix Metalloproteinase 2; MMP2; CLG4; CLG4A; MMP-II; MONA; TBE-1; Gelatinase A; MMP-2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes Human MMP-2 in samples
Assay Type
Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/ml
Precision
Coefficient of variation is<10%
Sensitivity
0.47ng/ml
Cross Reactivity
No significant cross-reactivity or interference between Human MMP-2 and analogues was observed
Detection Method
Absorbance: 450nm
Preparation and Storage
Store at 4 degree C
Shelf life: ~6 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP-2 elisa kit
The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. MMP-2 ELISA Kit is based on Sandwich ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human MMP-2. The concentration of Human MMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Product Categories/Family for MMP-2 elisa kit

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Product Notes

The Human MMP-2 (Catalog #AAA849429) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849429 ELISA Kit recognizes Human MMP-2. It is sometimes possible for the material contained within the vial of "MMP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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