Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) Standard Curve.)
Human Mitochondrial Import Receptor Subunit TOM5 Homolog (TOMM5) ELISA Kit | TOMM5 elisa kit
Human Mitochondrial Import Receptor Subunit TOM5 Homolog (TOMM5) ELISA Kit
Gene Names
TOMM5; Tom5; C9orf105; bA613M10.3
Reactivity
Human
Synonyms
Mitochondrial Import Receptor Subunit TOM5 Homolog (TOMM5); Human Mitochondrial Import Receptor Subunit TOM5 Homolog (TOMM5) ELISA Kit; TOMM5; RP11-613M10.3; C9orf105; RP11-263I4.1; Tom5; bA613M10.3; mitochondrial outer membrane protein TOM5; translocase of outer mitochondrial membrane 5 homolog; TOMM5 elisa kit
Reactivity
Human
Specificity
Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM5. No significant cross-reactivity or interference between Human TOMM5 and analogues was observed.
Sequence Length
51
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) Standard Curve.)
Related Product Information for TOMM5 elisa kit
Description: This Human Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM5 in samples. An antibody specific for TOMM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOMM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which regulate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These results indicate that human small Tom proteins maintain the structural integrity of the TOM complex.
Background: The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which regulate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These results indicate that human small Tom proteins maintain the structural integrity of the TOM complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial import receptor subunit TOM5 homolog isoform 1
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 5
NCBI Official Symbol
TOMM5
NCBI Official Synonym Symbols
Tom5; C9orf105; bA613M10.3
NCBI Protein Information
mitochondrial import receptor subunit TOM5 homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM5 homolog
Protein Family
UniProt Gene Name
TOMM5
UniProt Synonym Gene Names
C9orf105; TOM5
UniProt Entry Name
TOM5_HUMAN
Uniprot Description
TOMM5: Belongs to the Tom5 family.
Protein type: Membrane protein, integral; Mitochondrial; Transporter
Chromosomal Location of Human Ortholog: 9p13.2
Cellular Component: integral to membrane; mitochondrial outer membrane; mitochondrial outer membrane translocase complex; mitochondrion
Molecular Function: protein transporter activity
Biological Process: macroautophagy; protein targeting to mitochondrion
Research Articles on TOMM5
Similar Products
Product Notes
The Human TOMM5 tomm5 (Catalog #AAA9713829) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713829 ELISA Kit recognizes Human TOMM5. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Receptor Subunit TOM5 Homolog (TOMM5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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