Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial fission 1 protein (FIS1) Standard Curve.)
Human Mitochondrial Fission 1 Protein (FIS1) ELISA Kit | FIS1 elisa kit
Human Mitochondrial Fission 1 Protein (FIS1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial fission 1 protein (FIS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial fission 1 protein (FIS1) Standard Curve.)
Background: The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. The deduced 152-amino acid protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain. It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat. Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria. Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane. Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization.
NCBI and Uniprot Product Information
NCBI Description
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
Uniprot Description
FIS1: Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. Belongs to the FIS1 family.
Protein type: Membrane protein, integral; Mitochondrial
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: integral to peroxisomal membrane; protein complex; mitochondrion; membrane; peroxisome; integral to mitochondrial outer membrane
Molecular Function: protein binding; receptor binding
Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; elevation of cytosolic calcium ion concentration; mitochondrial fusion; reduction of endoplasmic reticulum calcium ion concentration; mitochondrion degradation; positive regulation of caspase activity; mitochondrial fragmentation during apoptosis; protein targeting to mitochondrion; protein homooligomerization; elevation of mitochondrial calcium ion concentration
Research Articles on FIS1
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Product Notes
The Human FIS1 fis1 (Catalog #AAA9715918) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715918 ELISA Kit recognizes Human FIS1. It is sometimes possible for the material contained within the vial of "Mitochondrial Fission 1 Protein (FIS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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