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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Microtubule-associated protein 1B (MAP1B) Standard Curve.)

Human Microtubule-Associated Protein 1B (MAP1B) ELISA Kit | MAP1B elisa kit

Human Microtubule-Associated Protein 1B (MAP1B) ELISA Kit

Gene Names
MAP1B; MAP5; FUTSCH; PPP1R102
Reactivity
Human
Synonyms
Microtubule-Associated Protein 1B (MAP1B); Human Microtubule-Associated Protein 1B (MAP1B) ELISA Kit; MAP1B; DKFZp686E1099; DKFZp686F1345; FLJ38954; FUTSCH; MAP5; MAP1B elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Microtubule-associated protein 1B (MAP1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAP1B. No significant cross-reactivity or interference between Human MAP1B and analogues was observed.
Sequence Length
2468
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Microtubule-associated protein 1B (MAP1B) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Microtubule-associated protein 1B (MAP1B) Standard Curve.)
Related Product Information for MAP1B elisa kit
Description: This Human Microtubule-associated protein 1B (MAP1B) ELISA Kit employs a two-site sandwich ELISA to quantitate MAP1B in samples. An antibody specific for MAP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MAP1B is a proteinbelongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. Two alternatively spliced transcript variants have been described.This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Microtubule-associated protein 1B
NCBI Official Synonym Full Names
microtubule associated protein 1B
NCBI Official Symbol
MAP1B
NCBI Official Synonym Symbols
MAP5; FUTSCH; PPP1R102
NCBI Protein Information
microtubule-associated protein 1B
UniProt Protein Name
Microtubule-associated protein 1B
Protein Family
UniProt Gene Name
MAP1B
UniProt Synonym Gene Names
MAP-1B
UniProt Entry Name
MAP1B_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

MAP1B: the function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: microtubule; microtubule associated complex; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; microtubule binding; structural molecule activity

Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of axon extension; dendrite development; cellular process; microtubule bundle formation; mitochondrion transport along microtubule

Research Articles on MAP1B

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Product Notes

The Human MAP1B map1b (Catalog #AAA9715408) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715408 ELISA Kit recognizes Human MAP1B. It is sometimes possible for the material contained within the vial of "Microtubule-Associated Protein 1B (MAP1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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