Human Metadherin (MTDH) ELISA Kit | MTDH elisa kit

Human Metadherin (MTDH) ELISA Kit

Cat. Num. AAA2704587

• Gene Names: MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• Reactivity: Human
• Synonyms: Metadherin (MTDH); Human Metadherin (MTDH) ELISA Kit; 3D3; AEG1; LYRIC; Astrocyte elevated gene-1 protein; Lysine-rich CEACAM1 co-isolated protein; Metastasis adhesion protein; MTDH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Metadherin (MTDH).; No significant cross-reactivity or interference between Metadherin (MTDH) and analogues was observed.
• Sequence Length: 582

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Metadherin (MTDH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Metadherin (MTDH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTDH elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metadherin (MTDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Metadherin (MTDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Metadherin (MTDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Metadherin (MTDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 28277147
• NCBI GeneID: 92140
• UniProt Accession #: Q86UE4; Q05DH2; Q52QU9; Q6PK07; Q8TCX3
• Molecular Weight: 63,837 Da
• NCBI Official Full Name: Metadherin
• NCBI Official Synonym Full Names: metadherin
• NCBI Official Symbol: MTDH
• NCBI Official Synonym Symbols: 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
• NCBI Protein Information: protein LYRIC
• UniProt Protein Name: Protein LYRIC
• Protein Family: Protein LYRIC
• UniProt Gene Name: MTDH
• UniProt Synonym Gene Names: AEG1; LYRIC; AEG-1

Uniprot Description

Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.