Human Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit | MED27 elisa kit

Human Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit

Cat. Num. AAA7203299

• Gene Names: MED27; CRSP8; CRAP34; CRSP34; TRAP37
• Reactivity: Human
• Synonyms: Mediator of RNA polymerase II transcription subunit 27 (MED27); Human Mediator of RNA polymerase II transcription subunit 27 (MED27) ELISA Kit; MED27 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Product Categories/Family for MED27 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 359751479
• NCBI GeneID: 9442
• NCBI Accession #: NP_001240810.1
• NCBI GenBank Nucleotide #: NM_001253881.1
• Molecular Weight: 14,826 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 27 isoform 2
• NCBI Official Synonym Full Names: mediator complex subunit 27
• NCBI Official Symbol: MED27
• NCBI Official Synonym Symbols: CRSP8; CRAP34; CRSP34; TRAP37
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 27; CRSP complex subunit 8; p37 TRAP/SMCC/PC2 subunit; transcriptional coactivator CRSP34; cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 27
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED27
• UniProt Synonym Gene Names: CRSP34; CRSP8; CRSP complex subunit 8
• UniProt Entry Name: MED27_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; Srb-mediator complex; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; stem cell maintenance; gene expression