Human Collagen Type IV Alpha 3 (COL4a3) RTU ELISA Kit | COL4a3 rtu elisa kit

Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit

Cat. Num. AAA458480

• Reactivity: Human
• Synonyms: Collagen Type IV Alpha 3 (COL4a3); Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit; COL4-A3; Tumstatin; Goodpasture Antigen; Collagen Alpha-3(IV)chain; COL4a3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1670

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.113ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4a3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to COL4a3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to COL4a3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4a3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL4a3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 89142730
• NCBI GeneID: 1285
• NCBI Accession #: NP_000082.2
• NCBI GenBank Nucleotide #: NM_000091.4
• UniProt Accession #: Q01955; Q53QQ1; Q53R14; Q53RW8; Q9BQT2; Q9NYC4; Q9UDJ9; Q9UDK9; Q9UDL0; Q9UDL1
• Molecular Weight: 135,079 Da
• NCBI Official Full Name: collagen alpha-3(IV) chain
• NCBI Official Synonym Full Names: collagen type IV alpha 3 chain
• NCBI Official Symbol: COL4A3
• NCBI Protein Information: collagen alpha-3(IV) chain
• UniProt Protein Name: Collagen alpha-3(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A3

NCBI Description

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.