Human MCM6 (minichromosome maintenance complex component 6) ELISA Kit | MCM6 elisa kit

Human MCM6 (minichromosome maintenance complex component 6) ELISA Kit

Cat. Num. AAA7606602

• Gene Names: MCM6; Mis5; P105MCM; MCG40308
• Reactivity: Human
• Synonyms: MCM6 (minichromosome maintenance complex component 6); Human MCM6 (minichromosome maintenance complex component 6) ELISA Kit; DNA replication licensing factor MCM6; P105mcm; MCM6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MCM6. No significant cross-reactivity or interference between MCM6 and analogues was observed.
• Sequence Length: 821

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 46.875-3000pg/ml
• Sensitivity: 28.125pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MCM6 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2497824
• NCBI GeneID: 4175
• UniProt Accession #: Q14566; Q13504; Q99859; B2R6H2
• Molecular Weight: 92,889 Da
• NCBI Official Full Name: DNA replication licensing factor MCM6
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 6
• NCBI Official Symbol: MCM6
• NCBI Official Synonym Symbols: Mis5; P105MCM; MCG40308
• NCBI Protein Information: DNA replication licensing factor MCM6
• UniProt Protein Name: DNA replication licensing factor MCM6
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM6

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]

Uniprot Description

MCM6: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Belongs to the MCM family.

Protein type: Cell cycle regulation; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: MCM complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus

Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; protein binding

Biological Process: DNA replication; G1/S transition of mitotic cell cycle

Disease: Lactose Intolerance, Adult Type