Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MARVEL domain-containing protein 2 (MARVELD2) Standard Curve.)
Human MARVEL Domain-Containing Protein 2 (MARVELD2) ELISA Kit | MARVELD2 elisa kit
Human MARVEL Domain-Containing Protein 2 (MARVELD2) ELISA Kit
Gene Names
MARVELD2; Tric; DFNB49; MARVD2; MRVLDC2
Reactivity
Human
Synonyms
MARVEL Domain-Containing Protein 2 (MARVELD2); Human MARVEL Domain-Containing Protein 2 (MARVELD2) ELISA Kit; DFNB49; FLJ30532; MARVD2; MRVLDC2; Tric; MARVEL (membrane-associating) domain containing 2; tricellulin; MARVELD2 elisa kit
Reactivity
Human
Specificity
Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MARVELD2. No significant cross-reactivity or interference between Human MARVELD2 and analogues was observed.
Sequence Length
558
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MARVEL domain-containing protein 2 (MARVELD2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human MARVEL domain-containing protein 2 (MARVELD2) Standard Curve.)
Related Product Information for MARVELD2 elisa kit
Description: This Human MARVEL domain-containing protein 2 (MARVELD2) ELISA Kit employs a two-site sandwich ELISA to quantitate MARVELD2 in samples. An antibody specific for MARVELD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MARVELD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARVELD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARVELD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts.The 555-amino acid protein contains cytoplasmic N and C termini, 2 transmembrane domains, and 2 extracellular loops. Northern blot analysis detected high expression of Tric in epithelium-derived tissues, including small intestine, kidney, and lung. Western blot analysis of mouse epithelial cell lines revealed an approximately 66-kD protein, as well as larger phosphorylated intracellular forms. Immunofluorescence microscopy showed expression of Tric predominantly at tricellular contacts where occludin (OCLN)-positive bicellular TJs converged. Tric-positive structures appeared as short rods through confocal microscopy.
Background: MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts.The 555-amino acid protein contains cytoplasmic N and C termini, 2 transmembrane domains, and 2 extracellular loops. Northern blot analysis detected high expression of Tric in epithelium-derived tissues, including small intestine, kidney, and lung. Western blot analysis of mouse epithelial cell lines revealed an approximately 66-kD protein, as well as larger phosphorylated intracellular forms. Immunofluorescence microscopy showed expression of Tric predominantly at tricellular contacts where occludin (OCLN)-positive bicellular TJs converged. Tric-positive structures appeared as short rods through confocal microscopy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
MARVEL domain-containing protein 2 isoform 1
NCBI Official Synonym Full Names
MARVEL domain containing 2
NCBI Official Symbol
MARVELD2
NCBI Official Synonym Symbols
Tric; DFNB49; MARVD2; MRVLDC2
NCBI Protein Information
MARVEL domain-containing protein 2
UniProt Protein Name
MARVEL domain-containing protein 2
Protein Family
UniProt Gene Name
MARVELD2
UniProt Synonym Gene Names
TRIC
UniProt Entry Name
MALD2_HUMAN
NCBI Description
The protein encoded by this gene is a membrane protein found at the tight junctions between epithelial cells. The encoded protein helps establish epithelial barriers such as those in the organ of Corti, where these barriers are required for normal hearing. Defects in this gene are a cause of deafness autosomal recessive type 49 (DFNB49). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Research Articles on MARVELD2
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Product Notes
The Human MARVELD2 marveld2 (Catalog #AAA9715385) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715385 ELISA Kit recognizes Human MARVELD2. It is sometimes possible for the material contained within the vial of "MARVEL Domain-Containing Protein 2 (MARVELD2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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