Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MGAT1 with C-terminal MYC tag probed with MBS422530 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat MGAT1 Polyclonal Antibody | anti-MGAT1 antibody
Goat anti-MGAT1 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Western Blot: In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx.55kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MGAT1 with C-terminal MYC tag probed with MBS422530 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MGAT1 with C-terminal MYC tag probed with MBS422530 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.
Protein type: EC 2.4.1.101; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Transferase
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: Golgi membrane; integral to membrane; membrane
Molecular Function: acetylglucosaminyltransferase activity; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity; metal ion binding
Biological Process: carbohydrate metabolic process; in utero embryonic development; protein amino acid glycosylation; UDP-N-acetylglucosamine catabolic process
Research Articles on MGAT1
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Product Notes
The MGAT1 mgat1 (Catalog #AAA422530) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MGAT1 Antibody reacts with Tested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MGAT1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx.55kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml. Researchers should empirically determine the suitability of the MGAT1 mgat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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