Western Blot (WB)
(Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.)
Rabbit Protein O-Fucosyltransferase 1 Polyclonal Antibody | anti-pofut1 antibody
Protein O-Fucosyltransferase 1 (POFUT1, FUT12, GDP-Fucose Protein O-Fucosyltransferase 1 Precursor, KIAA0180, MGC2482, O-Fuc-T, O-FucT-1, O-FUT, Peptide-O-fucosyltransferase, Peptide-O-fucosyltransferase 1)
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Protein O-Fucosyltransferase 1; Polyclonal Antibody; Protein O-Fucosyltransferase 1 (POFUT1; FUT12; GDP-Fucose Protein O-Fucosyltransferase 1 Precursor; KIAA0180; MGC2482; O-Fuc-T; O-FucT-1; O-FUT; Peptide-O-fucosyltransferase; Peptide-O-fucosyltransferase 1); Anti -Protein O-Fucosyltransferase 1 (POFUT1; anti-pofut1 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes human POFUT1. Species Crossreactivity: Mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS containing 0.02% sodium azide.
Applicable Applications for anti-pofut1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 0.5-1ug/ml
Dilution: Western Blot: 0.5-1ug/ml
Immunogen
Synthetic peptide corresponding to 16aa from near the amino terminus of human POFUT1.
Positive Control
Human Heart Tissue Lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.)
Western Blot (WB)
(Western blot analysis of POFUT1 in human heart tissue lysate with POFUT1 antibody at (A) 0.5 and (B) 1 ug/ml.)
Related Product Information for anti-pofut1 antibody
POFUT1, an endoplasmic reticulum-residing member of the glycosyltransferase O-Fuc family, adds O-fucose through an O-glycosidic linkage to conserved serine or threonines in epidermal growth factor-like repeats of several cell surface and secreted proteins. Unlike its homolog POFUT2, POFUT1 can also catalyze the transfer of fucose to thrombospondin type 1 repeats. Many of the substrates of POFUT1 are involved in ligand-induced receptor signaling. One such protein is Notch; mouse ES cells lacking POFUT have normal levels of Notch receptors at the cell surface, but these receptors do not bind Notch ligands or exhibit Notch signaling. At least two isoforms of POFUT1 are known to exist. This antibody is predicted to not cross-react with POFUT2.
Product Categories/Family for anti-pofut1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein O-fucosyltransferase 1
NCBI Official Symbol
pofut1
NCBI Protein Information
protein O-fucosyltransferase 1
Similar Products
Product Notes
The pofut1 (Catalog #AAA620923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Protein O-Fucosyltransferase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 0.5-1ug/ml. Researchers should empirically determine the suitability of the pofut1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein O-Fucosyltransferase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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