Human Mannosidase Alpha Class 2B Member 1 (MAN2B1) ELISA Kit | MAN2B1 elisa kit

Human Mannosidase Alpha Class 2B Member 1 (MAN2B1) ELISA Kit

Cat. Num. AAA2000160

• Gene Names: MAN2B1; MANB; LAMAN
• Reactivity: Human
• Synonyms: Mannosidase Alpha Class 2B Member 1 (MAN2B1); Human Mannosidase Alpha Class 2B Member 1 (MAN2B1) ELISA Kit; MANB; LAMAN; Lysosomal alpha-mannosidase; Lysosomal acid alpha-mannosidase; Mannosidase alpha-B; MAN2B1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 2B Member 1 (MAN2B1).; No significant cross-reactivity or interference between Mannosidase Alpha Class 2B Member 1 (MAN2B1) and analogues was observed.
• Sequence Length: 1010

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 14.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannosidase Alpha Class 2B Member 1 (MAN2B1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannosidase Alpha Class 2B Member 1 (MAN2B1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAN2B1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 2B Member 1 (MAN2B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 2B Member 1 (MAN2B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 2B Member 1 (MAN2B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAN2B1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119604685
• NCBI GeneID: 4125
• UniProt Accession #: O00754; O15330; Q16680; Q93094; Q9BW13; G5E928
• Molecular Weight: 113,616 Da
• NCBI Official Full Name: mannosidase, alpha, class 2B, member 1, isoform CRA_a
• NCBI Official Synonym Full Names: mannosidase, alpha, class 2B, member 1
• NCBI Official Symbol: MAN2B1
• NCBI Official Synonym Symbols: MANB; LAMAN
• NCBI Protein Information: lysosomal alpha-mannosidase
• UniProt Protein Name: Lysosomal alpha-mannosidase
• UniProt Gene Name: MAN2B1
• UniProt Synonym Gene Names: LAMAN; MANB; Laman
• UniProt Entry Name: MA2B1_HUMAN

NCBI Description

This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

MAN2B1: Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. Cleaves all known types of alpha-mannosidic linkages. Defects in MAN2B1 are the cause of lysosomal alpha- mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains. This accumulation is expressed histologically as cytoplasmic vacuolation predominantly in the CNS and parenchymatous organs. Depending on the clinical findings at the age of onset, a severe infantile (type I) and a mild juvenile (type II) form of alpha-mannosidosis are recognized. There is considerable variation in the clinical expression with mental retardation, recurrent infections, impaired hearing and Hurler- like skeletal changes being the most consistent abnormalities. Belongs to the glycosyl hydrolase 38 family.

Protein type: EC 3.2.1.24; Glycan Metabolism - other glycan degradation; Hydrolase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: lysosome

Molecular Function: zinc ion binding; carbohydrate binding; alpha-mannosidase activity

Biological Process: mannose metabolic process; protein deglycosylation; protein modification process

Disease: Mannosidosis, Alpha B, Lysosomal