Vascular Endothelial Growth Factor 165 (VEGF165) Recombinant Protein | VEGF165 recombinant protein

Recombinant Vascular Endothelial Growth Factor 165 (VEGF165)

Cat. Num. AAA2097669

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Reactivity: Human
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Vascular Endothelial Growth Factor 165 (VEGF165); Recombinant Vascular Endothelial Growth Factor 165 (VEGF165); VEGF165 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95%
• Form/Format: Freeze-dried powder
• Sequence Length: 412

• Applicable Applications for VEGF165 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Expression System: Prokaryotic expression
• Tag: N-terminal His Tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Fragment: Pro28~Asn141

SDS-Page

Product Categories/Family for VEGF165 recombinant protein

Cytokine; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 76781480
• NCBI GeneID: 7422
• NCBI Accession #: NP_001020537.2
• NCBI GenBank Nucleotide #: NM_001025366.2
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: Predicted Molecular Weight: 14.7kDa
• NCBI Official Full Name: vascular endothelial growth factor A isoform a
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().