Human Major histocompatibility complex I ELISA Kit | MHC 1 elisa kit

Human Major histocompatibility complex I ELISA Kit

Cat. Num. AAA724091

• Gene Names: HLA-B; AS; HLAB; SPDA1
• Reactivity: Human
• Synonyms: Major histocompatibility complex I; Human Major histocompatibility complex I ELISA Kit; MHC 1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This MHC 1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MHC 1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MHC 1 elisa kit

Principle of the Assay: MHC 1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MHC 1. Standards or samples are then added to the microtiter plate wells and MHC 1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MHC 1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for MHC 1 are added to each well to "sandwich" the MHC 1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MHC 1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MHC 1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MHC 1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1261808
• NCBI GeneID: 3106
• Molecular Weight: 40,460 Da
• NCBI Official Full Name: major histocompatibility complex
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; SPDA1
• NCBI Protein Information: major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, B-7 alpha chain
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B07_HUMAN

NCBI Description

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; integral to plasma membrane; early endosome membrane; endoplasmic reticulum; integral to membrane; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; regulation of interleukin-12 production; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of T cell anergy; regulation of interleukin-6 production; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; immune response