Human MAGEA1 (Melanoma-associated antigen 1) ELISA Kit | MAGEA1 elisa kit

Human MAGEA1 (Melanoma-associated antigen 1) ELISA Kit

Cat. Num. AAA7612361

• Gene Names: MAGEA1; CT1.1; MAGE1
• Reactivity: Human
• Synonyms: MAGEA1 (Melanoma-associated antigen 1); Human MAGEA1 (Melanoma-associated antigen 1) ELISA Kit; Melanoma-associated antigen 1; Antigen MZ2-E; Cancer/testis antigen 1.1; CT1.1; MAGE-1 antigen; MAGE1; MAGE1A; MAGEA1; MAGEA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAGEA1. No significant cross-reactivity or interference between MAGEA1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAGEA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148276977
• NCBI GeneID: 4100
• NCBI Accession #: NP_004979.3
• NCBI GenBank Nucleotide #: NM_004988.4
• UniProt Accession #: P43355; O00346; B2RC81; AAB33086
• Molecular Weight: 34,342 Da
• NCBI Official Full Name: melanoma-associated antigen 1
• NCBI Official Synonym Full Names: melanoma antigen family A1
• NCBI Official Symbol: MAGEA1
• NCBI Official Synonym Symbols: CT1.1; MAGE1
• NCBI Protein Information: melanoma-associated antigen 1; MAGE-1 antigen; antigen MZ2-E; cancer/testis antigen 1.1; cancer/testis antigen family 1, member 1; melanoma antigen MAGE-1; melanoma antigen family A 1; melanoma antigen family A, 1 (directs expression of antigen MZ2-E); me
• UniProt Protein Name: Melanoma-associated antigen 1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA1
• UniProt Synonym Gene Names: MAGE1; MAGE1A; CT1.1
• UniProt Entry Name: MAGA1_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-A1: a tumor antigen expressed in a variety of tumor cells and in the testis. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; histone deacetylase binding

Biological Process: transcription, DNA-dependent; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter