Human E2F Transcription Factor 3 (E2F3) ELISA Kit | E2F3 elisa kit

Human E2F Transcription Factor 3 (E2F3) ELISA Kit

Cat. Num. AAA454921

• Gene Names: E2F3; E2F-3
• Reactivity: Human
• Synonyms: E2F Transcription Factor 3 (E2F3); Human E2F Transcription Factor 3 (E2F3) ELISA Kit; E2F3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of E2F3. No significant cross-reactivity or interference between E2F3 and analogues was observed.
• Sequence Length: 334

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.072ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E2F3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E2F3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E2F3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of E2F3 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to E2F3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of E2F3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 340523141
• NCBI GeneID: 1871
• NCBI Accession #: NP_001230005.1
• NCBI GenBank Nucleotide #: NM_001243076.2
• UniProt Accession #: O00716; Q15000; Q68DT0; Q9BZ44
• Molecular Weight: 37,010 Da
• NCBI Official Full Name: transcription factor E2F3 isoform 2
• NCBI Official Synonym Full Names: E2F transcription factor 3
• NCBI Official Symbol: E2F3
• NCBI Official Synonym Symbols: E2F-3
• NCBI Protein Information: transcription factor E2F3
• UniProt Protein Name: Transcription factor E2F3
• Protein Family: Transcription factor
• UniProt Gene Name: E2F3
• UniProt Synonym Gene Names: KIAA0075; E2F-3

NCBI Description

This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters ().