Human Lysozyme, LZM ELISA Kit | LZM elisa kit

Human Lysozyme, LZM ELISA Kit

Cat. Num. AAA701900

• Gene Names: LYZ; LZM
• Reactivity: Human
• Synonyms: Lysozyme; LZM; Human Lysozyme; LZM ELISA Kit; LZM elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human LZM. No significant cross-reactivity or interference between Human LZM and analogues was observed.
• Sequence Length: 148

• Samples: Serum, plasma, tissue homogenates, urine
• Detection Range: 3.12 ng/ml-200 ng/ml
• Sensitivity: 0.78 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LZM elisa kit

Introduction: Lysozymes, also known as muramidase or N-acetylmuramide glycanhydrolase, are a family of enzymes which damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. It is abundant in a number of secretions, such as tears, saliva, and mucus. Lysozyme is also present in cytoplasmic granules of the polymorphonuclear neutrophils (PMN). Large amounts of lysozyme can be found in egg whites. C-type lysozymes are closely related to alpha-lactalbumin in sequence and structure making them part of the same family. In humans, the lysozyme enzyme is encoded by the LYZ gene. The enzyme functions by attacking peptidoglycans and hydrolyzing the glycosidic bond that connects N-acetylmuramic acid with the fourth carbon atom of N-acetylglucosamine. It does this by binding to the peptidoglycan molecule in the binding site within the prominent cleft between its two domains. This causes the substrate molecule to adopt a strained conformation similar to that of the transition state. According to Phillips-Mechanism the lysozyme binds to a hexasaccharide. The lysozyme then distorts the 4th sugar in hexasaccharide (the D ring) into a half-chair conformation. In this stressed state the glycosidic bond is easily broken. 3 The amino acid side chains glutamic acid 35 (Glu35) and aspartate 52 (Asp52) have been found to be critical to the activity of this enzyme. Glu35 acts as a proton donor to the glycosidic bond, cleaving the C-O bond in the substrate, whilst Asp52 acts as a nucleophile to generate a glycosyl enzyme intermediate. The glycosyl enzyme intermediate then reacts with a water molecule, to give the product of hydrolysis and leaving the enzyme unchanged.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated LZM and antibody preparation specific for LZM and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LZM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 307142
• NCBI GeneID: 4069
• UniProt Accession #: P61626; P00695; Q13170; Q9UCF8
• Molecular Weight: 16,537 Da
• NCBI Official Full Name: lysozyme (EC 3.2.1.17)
• NCBI Official Synonym Full Names: lysozyme
• NCBI Official Symbol: LYZ
• NCBI Official Synonym Symbols: LZM
• NCBI Protein Information: lysozyme C; c-type lysozyme; 1,4-beta-N-acetylmuramidase C
• UniProt Protein Name: Lysozyme C
• UniProt Gene Name: LYZ
• UniProt Synonym Gene Names: LZM
• UniProt Entry Name: LYSC_HUMAN

NCBI Description

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the anti-microbial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. Missense mutations in LYZ have been identified in heritable renal amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Catalytic activity: Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.