Rabbit MMP-22 Polyclonal Antibody | anti-MMP-22 antibody

Anti-Chicken MMP-22, Hinge Region Antibody

Cat. Num. AAA4157344

• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: MMP-22; Polyclonal Antibody; Anti-Chicken MMP-22; Hinge Region Antibody; anti-MMP-22 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody is specific for MMP22, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3, MMP-9, etc.).
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-MMP-22 antibody: Western Blot (WB)
• Application Notes: MMP-22 was first cloned from chicken fibroblasts, and named CMMP. Sequence comparisons confirmed CMMP was a metalloproteinase, but homology between chicken and human MMPs was too low to assign CMMP as a human ortholog. Several years later CMMP was given the moniker MMP-22. A BLAST of chicken MMP-22 finds human MMP-27 as the closest human sequence. Chicken MMP-22 compares most closely to human MMP-27, with 59% identity (compared to 51% identity between CMMP and human MMP-3). It is hazardous to guess the identity of species orthologs between chicken and human proteins: chicken MMP-2 is 84% identical to human and chicken MMP-13 is 75% identical to human, but chicken MMP-9 is only 59% identical to human MMP-9. The chicken MMP-22 sequence encodes a 472 amino acid protein, with predicted molecular weight of 53.254 kD. Although MMP-22 was thought to be most like human MMP-3 (Stromelysin-1), chicken MMP-22 protein has a quite basic predicted isoelectric point of 9.104, while human MMP-3 has a predicted isoelectric point of 5.699. Human MMP-27 has a predicted isoelectric point of 8.826, making it similar to CMMP. The signal sequence, and lack of a transmembrane domain lead to the assumption that MMP-22 is a secreted MMP, and it is tempting to speculate that the basic isoelectric point might direct the MMP-22 to interact with ECM. It is still uncertain if MMP-22 is a unique MMP, or the chicken ortholog of human MMP-27. RP3MMP-22 Anti-MMP-22 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 58 and 53 Kd, as well as smaller breakdown products_recover. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP-22 is well conserved, higher concentrations of antibody may be needed for non-human samples.
• Immunogen: Synthetic peptide based on the hinge region of the chicken MMP22 sequence.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-MMP-22 antibody

Description: The antibody is a Rabbit antibody to the Matrix Metalloproteinase MMP-22, made with a synthetic peptide based on the hinge region of the chicken sequence. The antibody binds to MMP-22, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3, MMP-9, etc.). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of sodium azide as preservative and 50% glycerol as cryoprotectant.

Relevance: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene lacks the signal sequence found in other MMPs, suggesting that it may function intracellularly. The substrate for the enzyme has not been identified, but it is thought that the protein may play a role in reproductive processes. This protein is encoded by the MMP22 or MMP23B gene which is part of a duplicated region of chromosome 1p36.3, which includes a second gene, MMP23A, which is almost identical. MMP22 was first cloned from chicken fibroblasts, and named CMMP. Sequence comparisons confirmed CMMP was a metalloproteinase, but homology between chicken and human MMPs was too low to assign CMMP as a human ortholog. Several years later CMMP was given the moniker MMP22. A BLAST of chicken MMP22 finds human MMP27 as the closest human sequence. Chicken MMP22 compares most closely to human MMP27, with 59% identity (compared to 51% identity between CMMP and human MMP3). It is hazardous to guess the identity of species orthologs between chicken and human proteins: chicken MMP2 is 84% identical to human and chicken MMP13 is 75% identical to human, but chicken MMP9 is only 59% identical to human MMP9. The chicken MMP22 sequence encodes a 472 amino acid protein, with predicted molecular weight of 53.254 kD. Although MMP22 was thought to be most like human MMP3 (Stromelysin 1), chicken MMP22 protein has a quite basic predicted isoelectric point of 9.104, while human MMP3 has a predicted isoelectric point of 5.699. Human MMP27 has a predicted isoelectric point of 8.826, making it similar to CMMP. The signal sequence, and lack of a transmembrane domain lead to the assumption that MMP22 is a secreted MMP, and it is tempting to speculate that the basic isoelectric point might direct the MMP22 to interact with ECM. It is still uncertain if MMP22 is a unique MMP, or the chicken ortholog of human MMP27.