Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lymphocyte antigen 96 (LY96) Standard Curve.)
Human Lymphocyte Antigen 96 (LY96) ELISA Kit | LY96 elisa kit
Human Lymphocyte Antigen 96 (LY96) ELISA Kit
Gene Names
LY96; MD2; MD-2; ly-96
Reactivity
Human
Synonyms
Lymphocyte Antigen 96 (LY96); Human Lymphocyte Antigen 96 (LY96) ELISA Kit; LY96; MD-2; MD2; MD-2 protein; myeloid differentiation protein-2; LY96 elisa kit
Reactivity
Human
Specificity
Human Lymphocyte antigen 96 (LY96) ELISA Kit has high sensitivity and excellent specificity for detection of Human LY96. No significant cross-reactivity or interference between Human LY96 and analogues was observed.
Sequence Length
160
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lymphocyte antigen 96 (LY96) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lymphocyte antigen 96 (LY96) Standard Curve.)
Related Product Information for LY96 elisa kit
Description: This Human Lymphocyte antigen 96 (LY96) ELISA Kit employs a two-site sandwich ELISA to quantitate LY96 in samples. An antibody specific for LY96 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LY96 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY96 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY96 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LY96 is involved in binding lipopolysaccharide with TLR 4. It is also known as "MD2". The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. MD2 did not interact with LY64, but it did appear on cells expressing Toll-like receptor-4 (TLR4). Toll-like receptors, which contain extracellular LRRs and have intracellular regions homologous to the intracellular signaling domain of the interleukin-1 receptor (IL1R), activate nuclear factor kappa-B (NFKB). TLR4 is involved in the response to lipopolysaccharide (LPS) from gram-negative bacteria. Functional analysis showed that expression of MD2 enhanced TLR4-dependent activation of NFKB.
Background: LY96 is involved in binding lipopolysaccharide with TLR 4. It is also known as "MD2". The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. MD2 did not interact with LY64, but it did appear on cells expressing Toll-like receptor-4 (TLR4). Toll-like receptors, which contain extracellular LRRs and have intracellular regions homologous to the intracellular signaling domain of the interleukin-1 receptor (IL1R), activate nuclear factor kappa-B (NFKB). TLR4 is involved in the response to lipopolysaccharide (LPS) from gram-negative bacteria. Functional analysis showed that expression of MD2 enhanced TLR4-dependent activation of NFKB.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Lymphocyte antigen 96
NCBI Official Synonym Full Names
lymphocyte antigen 96
NCBI Official Symbol
LY96
NCBI Official Synonym Symbols
MD2; MD-2; ly-96
NCBI Protein Information
lymphocyte antigen 96
UniProt Protein Name
Lymphocyte antigen 96
Protein Family
UniProt Gene Name
LY96
UniProt Synonym Gene Names
MD2; Ly-96
UniProt Entry Name
LY96_BOVIN
Similar Products
Product Notes
The Human LY96 ly96 (Catalog #AAA9715429) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715429 ELISA Kit recognizes Human LY96. It is sometimes possible for the material contained within the vial of "Lymphocyte Antigen 96 (LY96), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
