Human Lymphocyte Antigen 96 (LY96) ELISA Kit | LY96 elisa kit

Human Lymphocyte Antigen 96 (LY96) ELISA Kit

Cat. Num. AAA2706472

• Gene Names: Ly96; MD2; MD-2; ESOP-1
• Reactivity: Human
• Synonyms: Lymphocyte Antigen 96 (LY96); Human Lymphocyte Antigen 96 (LY96) ELISA Kit; MD-2; ESOP1; LY96 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lymphocyte Antigen 96 (LY96).; No significant cross-reactivity or interference between Lymphocyte Antigen 96 (LY96) and analogues was observed.
• Sequence Length: 160

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lymphocyte Antigen 96 (LY96) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lymphocyte Antigen 96 (LY96) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LY96 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Antigen 96 (LY96). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Antigen 96 (LY96). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Antigen 96 (LY96), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Antigen 96 (LY96) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393737
• NCBI GeneID: 17087
• NCBI Accession #: NP_058619.1
• NCBI GenBank Nucleotide #: NM_016923.2
• UniProt Accession #: Q9JHF9
• Molecular Weight: 18,394 Da
• NCBI Official Full Name: lymphocyte antigen 96 isoform A
• NCBI Official Synonym Full Names: lymphocyte antigen 96
• NCBI Official Symbol: Ly96
• NCBI Official Synonym Symbols: MD2; MD-2; ESOP-1
• NCBI Protein Information: lymphocyte antigen 96
• UniProt Protein Name: Lymphocyte antigen 96
• Protein Family: Lymphocyte antigen
• UniProt Gene Name: Ly96
• UniProt Synonym Gene Names: Esop1; Md2; Ly-96

Uniprot Description

Binds bacterial lipopolysaccharide (LPS) (PubMed:22532668). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10725698).