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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TCOF1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human, Mouse TCOF1 Polyclonal Antibody | anti-TCOF1 antibody

TCOF1 Antibody

Gene Names
TCOF1; TCS; MFD1; TCS1; treacle
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
TCOF1; Polyclonal Antibody; TCOF1 Antibody; anti-TCOF1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
TCOF1 Antibody detects endogenous levels of total TCOF1 protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1,412
Applicable Applications for anti-TCOF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human TCOF1.
Immunogen Range
41-90
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TCOF1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TCOF1 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat, 293, HeLa cells, using TCOF1 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from Jurkat, 293, HeLa cells, using TCOF1 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
152 kDa
NCBI Official Full Name
treacle protein isoform b
NCBI Official Synonym Full Names
treacle ribosome biogenesis factor 1
NCBI Official Symbol
TCOF1
NCBI Official Synonym Symbols
TCS; MFD1; TCS1; treacle
NCBI Protein Information
treacle protein
UniProt Protein Name
Treacle protein
Protein Family
UniProt Gene Name
TCOF1

NCBI Description

This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).

Research Articles on TCOF1

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Product Notes

The TCOF1 tcof1 (Catalog #AAA9505202) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCOF1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TCOF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the TCOF1 tcof1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCOF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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