Human Lipase, Monoacylglycerol (MGL) ELISA Kit | MGL elisa kit

Human Lipase, Monoacylglycerol (MGL) ELISA Kit

Cat. Num. AAA2024623

• Gene Names: MGLL; MGL; HUK5; MAGL; HU-K5
• Reactivity: Human
• Synonyms: Lipase; Monoacylglycerol (MGL); Human Lipase; Monoacylglycerol (MGL) ELISA Kit; HU-K5; MGLL; MAGL; Monoglyceride Lipase; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MGL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipase, Monoacylglycerol (MGL).; No significant cross-reactivity or interference between Lipase, Monoacylglycerol (MGL) and analogues was observed.
• Sequence Length: 283

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MGL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Monoacylglycerol (MGL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Monoacylglycerol (MGL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Monoacylglycerol (MGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Monoacylglycerol (MGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MGL elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 375268702
• NCBI GeneID: 11343
• NCBI Accession #: NP_001243514.1
• NCBI GenBank Nucleotide #: NM_001256585.1
• UniProt Accession #: Q99685; Q6IBG9; Q96AA5; B3KRC2; B7Z9D1
• Molecular Weight: 31,149 Da
• NCBI Official Full Name: monoglyceride lipase isoform 3
• NCBI Official Synonym Full Names: monoglyceride lipase
• NCBI Official Symbol: MGLL
• NCBI Official Synonym Symbols: MGL; HUK5; MAGL; HU-K5
• NCBI Protein Information: monoglyceride lipase
• UniProt Protein Name: Monoglyceride lipase
• Protein Family: Methionine gamma-lyase
• UniProt Gene Name: MGLL
• UniProt Synonym Gene Names: MAGL
• UniProt Entry Name: MGLL_HUMAN

NCBI Description

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

Protein type: Lipid Metabolism - glycerolipid; EC 3.1.1.23; Phospholipase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol

Molecular Function: protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity

Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; regulation of inflammatory response; triacylglycerol catabolic process; arachidonic acid metabolic process; regulation of sensory perception of pain; lipid metabolic process; inflammatory response; blood coagulation; fatty acid biosynthetic process; acylglycerol catabolic process