Rabbit anti-Human, Mouse Cyclin C Polyclonal Antibody | anti-CCNC antibody

Cyclin C (Ab-275) Antibody

Cat. Num. AAA9401942

• Gene Names: CCNC; CycC
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Cyclin C; Polyclonal Antibody; Cyclin C (Ab-275) Antibody; The antiserum was produced against synthesized peptide derived from human Cyclin C.; anti-CCNC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total Cyclin C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 198

• Applicable Applications for anti-CCNC antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human Cyclin C around the phosphorylation site of serine 275 (N-G-S(p)-Q-N).
• Target Name: Cyclin C
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from LOVO cells, using Cyclin C (Ab-275) antibody.)

Related Product Information for anti-CCNC antibody

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.Lew D.J., Cell 66:1197-1206(1991).Li H., Genomics 32:253-259(1996).Mungall A.J., Nature 425:805-811(2003).

Product Categories/Family for anti-CCNC antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 61676093
• NCBI GeneID: 892
• NCBI Accession #: NP_001013417.1
• NCBI GenBank Nucleotide #: NM_001013399.1
• UniProt Accession #: P24863; Q9H543; B4DPZ1
• Molecular Weight: 22,945 Da
• NCBI Official Full Name: cyclin-C isoform b
• NCBI Official Synonym Full Names: cyclin C
• NCBI Official Symbol: CCNC
• NCBI Official Synonym Symbols: CycC
• NCBI Protein Information: cyclin-C
• UniProt Protein Name: Cyclin-C
• Protein Family: Cyclin
• UniProt Gene Name: CCNC
• UniProt Synonym Gene Names: hSRB11
• UniProt Entry Name: CCNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNC: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, holoenzyme; Srb-mediator complex

Molecular Function: protein binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of cyclin-dependent protein kinase activity