Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LIM domain transcription factor LMO4 (LMO4) Standard Curve.)
Human LIM Domain Transcription Factor LMO4 (LMO4) ELISA Kit | LMO4 elisa kit
Human LIM Domain Transcription Factor LMO4 (LMO4) ELISA Kit
Reactivity
Human
Synonyms
LIM Domain Transcription Factor LMO4 (LMO4); Human LIM Domain Transcription Factor LMO4 (LMO4) ELISA Kit; LMO4; OTTHUMP00000011906; LMO4 elisa kit
Reactivity
Human
Specificity
Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LMO4. No significant cross-reactivity or interference between Human LMO4 and analogues was observed.
Sequence Length
165
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LIM domain transcription factor LMO4 (LMO4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human LIM domain transcription factor LMO4 (LMO4) Standard Curve.)
Related Product Information for LMO4 elisa kit
Description: This Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit employs a two-site sandwich ELISA to quantitate LMO4 in samples. An antibody specific for LMO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LMO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMO4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LMO4 was expressed predominantly in the lobuloalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LMO4 mRNA was observed in 5 of 10 human breast cancer cell lines. Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of specimens. Immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies implied a role for LMO4 in maintaining proliferation of mammary epithelium and suggested that deregulation of this gene may contribute to breast tumorigenesis.
Background: LMO4 was expressed predominantly in the lobuloalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LMO4 mRNA was observed in 5 of 10 human breast cancer cell lines. Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of specimens. Immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies implied a role for LMO4 in maintaining proliferation of mammary epithelium and suggested that deregulation of this gene may contribute to breast tumorigenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
LIM domain transcription factor LMO4
NCBI Official Synonym Full Names
LIM domain only 4
NCBI Official Symbol
LMO4
NCBI Protein Information
LIM domain transcription factor LMO4
UniProt Protein Name
LIM domain transcription factor LMO4
Protein Family
UniProt Gene Name
LMO4
UniProt Synonym Gene Names
LMO-4
UniProt Entry Name
LMO4_HUMAN
NCBI Description
This gene encodes a cysteine-rich protein that contains two LIM domains but lacks a DNA-binding homeodomain. The encoded protein may play a role as a transcriptional regulator or as an oncogene. [provided by RefSeq, Aug 2008]
Uniprot Description
LMO4: Probable transcriptional factor.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 1p22.3
Cellular Component: transcription factor complex
Molecular Function: zinc ion binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; thymus development; ventral spinal cord interneuron differentiation; neural tube closure; regulation of cell activation; regulation of cell fate specification; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; spinal cord motor neuron differentiation; negative regulation of protein complex assembly
Research Articles on LMO4
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Product Notes
The Human LMO4 lmo4 (Catalog #AAA9716037) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716037 ELISA Kit recognizes Human LMO4. It is sometimes possible for the material contained within the vial of "LIM Domain Transcription Factor LMO4 (LMO4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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