Rabbit anti-Human, Mouse PSME2 Polyclonal Antibody | anti-PSME2 antibody

PSME2, ID (Proteasome Activator Complex Subunit 2, Proteasome Activator 28 Subunit beta, PA28beta, PA28b, Activator Of Multicatalytic Protease Subunit 2, 11S Regulator Complex Subunit beta, REG-beta) (FITC)

Cat. Num. AAA6494935

• Gene Names: PSME2; PA28B; REGbeta; PA28beta
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography
• Synonyms: PSME2; Polyclonal Antibody; ID (Proteasome Activator Complex Subunit 2; Proteasome Activator 28 Subunit beta; PA28beta; PA28b; Activator Of Multicatalytic Protease Subunit 2; 11S Regulator Complex Subunit beta; REG-beta) (FITC); anti-PSME2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSME2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PSME2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; FLISA: 1:1,000; IHC: 1:50-1:100; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide between a portion within aa69-98 in the Center region of human PSME2
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PSME2 Antibody (Center) in HL-60,Hela cell line and mouse liver tissue lysates (35ug/lane).PSME2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded mouse hepatocarcinoma reacted with PSME2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PSME2 Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PSME2 antibody

Antibodies; Proteasomes

References

1. Conticello, S.G., Curr. Biol. 13(22), 2009-2013 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 30410792
• NCBI GeneID: 5721
• NCBI Accession #: NP_002809
• NCBI GenBank Nucleotide #: NM_002818
• UniProt Accession #: Q9UL46
• Molecular Weight: 27kDa
• NCBI Official Full Name: proteasome activator complex subunit 2
• NCBI Official Synonym Full Names: proteasome activator subunit 2
• NCBI Official Symbol: PSME2
• NCBI Official Synonym Symbols: PA28B; REGbeta; PA28beta
• NCBI Protein Information: proteasome activator complex subunit 2
• UniProt Protein Name: Proteasome activator complex subunit 2
• Protein Family: Proteasome activator complex
• UniProt Gene Name: PSME2
• UniProt Synonym Gene Names: REG-beta; PA28b; PA28beta
• UniProt Entry Name: PSME2_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

PSME2: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. By IFNG/IFN-gamma. Belongs to the PA28 family.

Protein type: Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; membrane; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle