Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) Standard Curve.)
Human Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 (LRRK2) ELISA Kit | LRRK2 elisa kit
Human Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 (LRRK2) ELISA Kit
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human
Synonyms
Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 (LRRK2); Human Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 (LRRK2) ELISA Kit; LRRK2; AURA17; DARDARIN; PARK8; RIPK7; ROCO2; augmented in rheumatoid arthritis 17; LRRK2 elisa kit
Reactivity
Human
Specificity
Human Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRRK2. No significant cross-reactivity or interference between Human LRRK2 and analogues was observed.
Sequence Length
2527
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) Standard Curve.)
Related Product Information for LRRK2 elisa kit
Description: This Human Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRK2 in samples. An antibody specific for LRRK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRRK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LRRK2 encodes a protein with an ankyrin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, an MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.LRRK2 interacts with the C-terminal R2 RING finger domain of parkin, and parkin interacted with the COR domain of LRRK2. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. Mutations in this gene have been associated with Parkinson's disease type 8.Four LRRK2 gene variants found are found in a third of Parkinson's cases, but infrequent in the general population.
Background: LRRK2 encodes a protein with an ankyrin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, an MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane.LRRK2 interacts with the C-terminal R2 RING finger domain of parkin, and parkin interacted with the COR domain of LRRK2. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons. Mutations in this gene have been associated with Parkinson's disease type 8.Four LRRK2 gene variants found are found in a third of Parkinson's cases, but infrequent in the general population.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
leucine-rich repeat serine/threonine-protein kinase 2
NCBI Official Synonym Full Names
leucine rich repeat kinase 2
NCBI Official Symbol
LRRK2
NCBI Official Synonym Symbols
PARK8; RIPK7; ROCO2; AURA17; DARDARIN
NCBI Protein Information
leucine-rich repeat serine/threonine-protein kinase 2
UniProt Protein Name
Leucine-rich repeat serine/threonine-protein kinase 2
Protein Family
UniProt Gene Name
LRRK2
UniProt Synonym Gene Names
PARK8
UniProt Entry Name
LRRK2_HUMAN
NCBI Description
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]
Research Articles on LRRK2
Similar Products
Product Notes
The Human LRRK2 lrrk2 (Catalog #AAA9715440) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715440 ELISA Kit recognizes Human LRRK2. It is sometimes possible for the material contained within the vial of "Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2 (LRRK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
