Human Leucine-Rich Repeat, Immunoglobulin-Like Domain And Transmembrane Domain-Containing Protein 3 (LRIT3) ELISA Kit | LRIT3 elisa kit

Human Leucine-Rich Repeat, Immunoglobulin-Like Domain And Transmembrane Domain-Containing Protein 3 (LRIT3) ELISA Kit

Cat. Num. AAA9716035

• Gene Names: LRIT3; CSNB1F; FIGLER4
• Reactivity: Human
• Synonyms: Leucine-Rich Repeat; Immunoglobulin-Like Domain And Transmembrane Domain-Containing Protein 3 (LRIT3); Human Leucine-Rich Repeat; Immunoglobulin-Like Domain And Transmembrane Domain-Containing Protein 3 (LRIT3) ELISA Kit; LRIT3; FIGLER4; FLJ44691; MGC120618; fibronectin typeI; immunoglobulin and leucine rich repeat domains 4; LRIT3 elisa kit

• Reactivity: Human
• Specificity: Human Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 (LRIT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRIT3. No significant cross-reactivity or interference between Human LRIT3 and analogues was observed.
• Sequence Length: 679

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 (LRIT3) Standard Curve.)

Related Product Information for LRIT3 elisa kit

Description: This Human Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3 (LRIT3) ELISA Kit employs a two-site sandwich ELISA to quantitate LRIT3 in samples. An antibody specific for LRIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: LRIT3 Contains 1 fibronectin type-III domain.Contains 1 Ig-like (immunoglobulin-like) domain.Contains 4 LRR (leucine-rich) repeats.Contains 1 LRRCT domain. A leucine-rich repeat (LRR) is a protein structural motif that forms an alpha/beta horseshoe fold.[1][2] It is composed of repeating 20-30 amino acid stretches that are unusually rich in the hydrophobic amino acid leucine. Typically, each repeat unit has beta strand-turn-alpha helix structure, and the assembled domain, composed of many such repeats, has a horseshoe shape with an interior parallel beta sheet and an exterior array of helices. One face of the beta sheet and one side of the helix array are exposed to solvent and are therefore dominated by hydrophilic residues.

NCBI and Uniprot Product Information

• NCBI GI #: 385298685
• NCBI GeneID: 345193
• NCBI Accession #: NP_940908.3
• NCBI GenBank Nucleotide #: NP_940908.3
• UniProt Accession #: Q3SXY7
• NCBI Official Full Name: leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3
• NCBI Official Synonym Full Names: leucine rich repeat, Ig-like and transmembrane domains 3
• NCBI Official Symbol: LRIT3
• NCBI Official Synonym Symbols: CSNB1F; FIGLER4
• NCBI Protein Information: leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3
• UniProt Protein Name: Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein 3
• Protein Family: Leucine-rich repeat, immunoglobulin-like domain and transmembrane domain-containing protein
• UniProt Gene Name: LRIT3
• UniProt Entry Name: LRIT3_HUMAN

NCBI Description

This gene encodes a protein that has a fibronectin type III domain and a C-terminal transmembrane domain, as well as a leucine-rich repeat domain and immunoglobulin-like domain near the N-terminus. The encoded protein may regulate fibroblast growth factor receptors and affect the modification of these receptors, which are glycosylated differently in the Golgi and endoplasmic reticulum. Mutations in this gene are associated with congenital stationary night blindness, type 1F. [provided by RefSeq, May 2013]

Uniprot Description

LRIT3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Biological Process: regulation of fibroblast growth factor receptor signaling pathway

Disease: Night Blindness, Congenital Stationary, Type 1f