Human LETM1 Domain Containing Protein 1 (LETMD1) ELISA Kit | LETMD1 elisa kit

Human LETM1 Domain Containing Protein 1 (LETMD1) ELISA Kit

Cat. Num. AAA2707287

• Gene Names: LETM1; SLC55A1
• Reactivity: Human
• Synonyms: LETM1 Domain Containing Protein 1 (LETMD1); Human LETM1 Domain Containing Protein 1 (LETMD1) ELISA Kit; HCCR1; HCRR-2; Cervical Cancer 1 Protooncogene; Cervical cancer proto-oncogene 2 protein; LETMD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LETM1 Domain Containing Protein 1 (LETMD1).; No significant cross-reactivity or interference between LETM1 Domain Containing Protein 1 (LETMD1) and analogues was observed.
• Sequence Length: 739

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LETM1 Domain Containing Protein 1 (LETMD1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LETM1 Domain Containing Protein 1 (LETMD1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LETMD1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LETM1 Domain Containing Protein 1 (LETMD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LETM1 Domain Containing Protein 1 (LETMD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LETM1 Domain Containing Protein 1 (LETMD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LETM1 Domain Containing Protein 1 (LETMD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6912482
• NCBI GeneID: 3954
• NCBI Accession #: NP_036450.1
• NCBI GenBank Nucleotide #: NM_012318.2
• UniProt Accession #: O95202; Q9UF65; B4DED2
• Molecular Weight: 33,245 Da
• NCBI Official Full Name: mitochondrial proton/calcium exchanger protein
• NCBI Official Synonym Full Names: leucine zipper and EF-hand containing transmembrane protein 1
• NCBI Official Symbol: LETM1
• NCBI Official Synonym Symbols: SLC55A1
• NCBI Protein Information: mitochondrial proton/calcium exchanger protein; LETM1 and EF-hand domain-containing protein 1, mitochondrial
• UniProt Protein Name: Mitochondrial proton/calcium exchanger protein
• Protein Family: LETM1 domain-containing protein
• UniProt Gene Name: LETM1

NCBI Description

This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]

Uniprot Description

LETM1: Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: integral component of membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: calcium ion binding; calcium:hydrogen antiporter activity; protein binding; ribosome binding

Biological Process: cellular metal ion homeostasis; cristae formation; mitochondrial calcium ion homeostasis; mitochondrial calcium ion transport; protein homooligomerization

Disease: Wolf-hirschhorn Syndrome