Human Laminin Alpha 3 (LAMa3) ELISA Kit | LAMa3 elisa kit

Human Laminin Alpha 3 (LAMa3) ELISA Kit

Cat. Num. AAA2021648

• Gene Names: LAMA3; E170; LOCS; BM600; LAMNA
• Reactivity: Human
• Synonyms: Laminin Alpha 3 (LAMa3); Human Laminin Alpha 3 (LAMa3) ELISA Kit; LAM-A3; E170; LAMNA; LOCS; Lama3a; Nicein subunit alpha; Kalinin subunit alpha; Epiligrin subunit alpha; LAMa3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Laminin Alpha 3 (LAMa3).; No significant cross-reactivity or interference between Laminin Alpha 3 (LAMa3) and analogues was observed.
• Sequence Length: 1,668

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.0pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 3 (LAMa3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 3 (LAMa3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMa3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 3 (LAMa3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 3 (LAMa3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 3 (LAMa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 3 (LAMa3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LAMa3 elisa kit

Signal transduction; Metabolic pathway; Tumor immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 34452237
• NCBI GeneID: 3909
• UniProt Accession #: Q16787; Q13679; Q13680; Q6VU67; Q6VU68; Q6VU69; Q76E14; Q96TG0; B0YJ33
• Molecular Weight: 184,054 Da
• NCBI Official Full Name: laminin alpha 3 splice variant b1
• NCBI Official Synonym Full Names: laminin, alpha 3
• NCBI Official Symbol: LAMA3
• NCBI Official Synonym Symbols: E170; LOCS; BM600; LAMNA
• NCBI Protein Information: laminin subunit alpha-3
• UniProt Protein Name: Laminin subunit alpha-3
• Protein Family: Laminin
• UniProt Gene Name: LAMA3
• UniProt Synonym Gene Names: LAMNA; E170
• UniProt Entry Name: LAMA3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane

Molecular Function: structural molecule activity; receptor binding

Biological Process: regulation of cell adhesion; extracellular matrix disassembly; epidermis development; hemidesmosome assembly; extracellular matrix organization and biogenesis; regulation of embryonic development; cell adhesion; regulation of cell migration

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type