Human Epiligrin ELISA Kit | EP elisa kit

Human Epiligrin ELISA Kit

Cat. Num. AAA720140

• Gene Names: LAMA3; E170; LOCS; BM600; LAMNA; lama3a
• Reactivity: Human
• Synonyms: Epiligrin; Human Epiligrin ELISA Kit; EP elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EP elisa kit

Intended Uses: This EP ELISA kit is intended Laboratory for research use only. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofEP in the sample, thisEP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusEP concentration. The concentration ofEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This EP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for EP. Standards or samples are then added to the microtiter plate wells and EP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forEP are added to each well to "sandwich" the EP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containEP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 551599
• NCBI GeneID: 3909
• Molecular Weight: 184,054 Da
• NCBI Official Full Name: epiligrin alpha 3 subunit, partial
• NCBI Official Synonym Full Names: laminin, alpha 3
• NCBI Official Symbol: LAMA3
• NCBI Official Synonym Symbols: E170; LOCS; BM600; LAMNA; lama3a
• NCBI Protein Information: laminin subunit alpha-3; BM600 150kD subunit; nicein 150kD subunit; nicein subunit alpha; kalinin 165kD subunit; kalinin subunit alpha; epiligrin subunit alpha; laminin-5 alpha 3 chain; laminin-5 subunit alpha; laminin-6 subunit alpha; laminin-7 subunit alpha; epiligrin 170 kda subunit; epiligrin alpha 3 subunit; laminin, alpha 3 (nicein (150kD), kalinin (165kD), BM600 (150kD), epilegrin)
• UniProt Protein Name: Laminin subunit alpha-3
• UniProt Gene Name: LAMA3
• UniProt Synonym Gene Names: LAMNA; E170
• UniProt Entry Name: LAMA3_HUMAN

NCBI Description

Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. The protein encoded by this gene is the alpha-3 subunit of laminin 5, which is a complex glycoprotein composed of three subunits (alpha, beta, and gamma). Laminin 5 is thought to be involved in cell adhesion, signal transduction and differentiation of keratinocytes. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMA3: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMA3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. Defects in LAMA3 are the cause of laryngoonychocutaneous syndrome (LOCS). LOCS is an autosomal recessive epithelial disorder confined to the Punjabi Muslim population. The condition is characterized by cutaneous erosions, nail dystrophy and exuberant vascular granulation tissue in certain epithelia, especially conjunctiva and larynx. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: laminin-5 complex; laminin-1 complex; extracellular region; basement membrane

Molecular Function: structural molecule activity; receptor binding

Biological Process: regulation of cell adhesion; extracellular matrix disassembly; hemidesmosome assembly; extracellular matrix organization and biogenesis; epidermis development; regulation of embryonic development; cell adhesion; regulation of cell migration

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Laryngoonychocutaneous Syndrome; Epidermolysis Bullosa, Junctional, Herlitz Type