Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) Standard Curve.)
Human Kynurenine/alpha-Aminoadipate Aminotransferase, Mitochondrial (AADAT) ELISA Kit | AADAT elisa kit
Human Kynurenine/alpha-Aminoadipate Aminotransferase, Mitochondrial (AADAT) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (AADAT) Standard Curve.)
Background: Aminoadipate Aminotransferase is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist. Higher expression in the liver. Also found in heart, brain, kidney, pancreas, prostate, testis and ovary.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Uniprot Description
AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.6.1.7; Amino Acid Metabolism - tryptophan; EC 2.6.1.39; Amino Acid Metabolism - lysine degradation; Transferase; Amino Acid Metabolism - lysine biosynthesis; Mitochondrial
Chromosomal Location of Human Ortholog: 4q33
Cellular Component: mitochondrial matrix
Molecular Function: protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; 2-aminoadipate transaminase activity; pyridoxal phosphate binding
Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process; biosynthetic process; tryptophan catabolic process; 2-oxoglutarate metabolic process
Research Articles on AADAT
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Product Notes
The Human AADAT aadat (Catalog #AAA9714509) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714509 ELISA Kit recognizes Human AADAT. It is sometimes possible for the material contained within the vial of "Kynurenine/alpha-Aminoadipate Aminotransferase, Mitochondrial (AADAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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