Mouse Bone Morphogenetic Protein 10 (BMP10) ELISA Kit | BMP10 elisa kit

Mouse Bone Morphogenetic Protein 10 (BMP10) ELISA Kit

Cat. Num. AAA2018921

• Reactivity: Mouse
• Synonyms: Bone Morphogenetic Protein 10 (BMP10); Mouse Bone Morphogenetic Protein 10 (BMP10) ELISA Kit; BMP10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 10 (BMP10).; No significant cross-reactivity or interference between Bone Morphogenetic Protein 10 (BMP10) and analogues was observed.
• Sequence Length: 424

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 10 (BMP10) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 10 (BMP10) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP10 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 10 (BMP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 10 (BMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 10 (BMP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 10 (BMP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BMP10 elisa kit

Cardiovascular biology; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 7656928
• NCBI GeneID: 27302
• NCBI Accession #: NP_055297.1
• NCBI GenBank Nucleotide #: NM_014482.1
• UniProt Accession #: O95393; Q53R17; Q6NTE0
• Molecular Weight: 48,047 Da
• NCBI Official Full Name: bone morphogenetic protein 10 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 10
• NCBI Official Symbol: BMP10
• NCBI Protein Information: bone morphogenetic protein 10
• UniProt Protein Name: Bone morphogenetic protein 10
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP10
• UniProt Synonym Gene Names: BMP-10
• UniProt Entry Name: BMP10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta family of growth factors. Data suggest that the similar protein in mouse plays an important role in trabeculation of the embryonic heart. In human, this protein may signal through receptor serine/threonine kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP10: Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; Z disc

Molecular Function: receptor serine/threonine kinase binding; protein binding; growth factor activity; telethonin binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: adult heart development; extracellular matrix organization and biogenesis; atrial cardiac muscle morphogenesis; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; activin receptor signaling pathway; cardiac muscle cell proliferation; sarcomere organization; BMP signaling pathway; regulation of apoptosis; regulation of MAPKKK cascade; ventricular cardiac muscle morphogenesis; negative regulation of cell growth; cell adhesion; negative regulation of cell migration; positive regulation of cardiac muscle cell proliferation