Human Krueppel-like factor 5 (KLF5) ELISA Kit | KLF5 elisa kit

Human Krueppel-like factor 5 (KLF5) ELISA Kit

Cat. Num. AAA2802168

• Gene Names: KLF5; CKLF; IKLF; BTEB2
• Reactivity: Human
• Synonyms: Krueppel-like factor 5 (KLF5); Human Krueppel-like factor 5 (KLF5) ELISA Kit; BTEB2; CKLF; IKLF; GC box binding protein 2|Kruppel-like factor 5|basic transcription element binding protein 2|colon kruppel-like factor|intestinal-enriched kruppel-like factor|transcription factor; KLF5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human KLF5. No significant cross-reactivity or interference between Human KLF5 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate KLF5 in samples. An antibody specific for KLF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 12644412
• NCBI GeneID: 688
• UniProt Accession #: Q13887; Q9UHP8; L0R3U5; L0R4T9
• Molecular Weight: 457
• NCBI Official Full Name: Krueppel-like factor 5
• NCBI Official Synonym Full Names: Kruppel-like factor 5 (intestinal)
• NCBI Official Symbol: KLF5
• NCBI Official Synonym Symbols: CKLF; IKLF; BTEB2
• NCBI Protein Information: Krueppel-like factor 5; Klf5C isoform; BTE-binding protein 2; GC box binding protein 2; colon kruppel-like factor; colon krueppel-like factor; transcription factor BTEB2; intestinal-enriched kruppel-like factor; intestinal-enriched krueppel-like factor; b
• UniProt Protein Name: Krueppel-like factor 5
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF5
• UniProt Synonym Gene Names: BTEB2; CKLF; IKLF; BTE-binding protein 2
• UniProt Entry Name: KLF5_HUMAN

NCBI Description

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane

Molecular Function: protein binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of microvillus biogenesis