Rabbit KLF5 Polyclonal Antibody | anti-KLF5 antibody

KLF5 (Kruppel-like Factor 5, CKLF, IKLF, BTEB3) (Biotin)

Cat. Num. AAA6424012

• Gene Names: KLF5; CKLF; IKLF; BTEB2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: KLF5; Polyclonal Antibody; KLF5 (Kruppel-like Factor 5; CKLF; IKLF; BTEB3) (Biotin); anti-KLF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes KLF5. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-KLF5 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:200-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human KLF5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using KLF5 antibody. (1: ROMAS cell lysate 2: HO-8910 cell lysate 3: HCT116 cell lysate))

Product Categories/Family for anti-KLF5 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 12644412
• NCBI GeneID: 688
• UniProt Accession #: Q13887; Q9UHP8; L0R3U5; L0R4T9
• Molecular Weight: 457
• NCBI Official Full Name: Krueppel-like factor 5
• NCBI Official Synonym Full Names: Kruppel-like factor 5 (intestinal)
• NCBI Official Symbol: KLF5
• NCBI Official Synonym Symbols: CKLF; IKLF; BTEB2
• NCBI Protein Information: Krueppel-like factor 5; Klf5C isoform; BTE-binding protein 2; GC box binding protein 2; colon kruppel-like factor; colon krueppel-like factor; transcription factor BTEB2; intestinal-enriched kruppel-like factor; intestinal-enriched krueppel-like factor; b
• UniProt Protein Name: Krueppel-like factor 5
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF5
• UniProt Synonym Gene Names: BTEB2; CKLF; IKLF; BTE-binding protein 2
• UniProt Entry Name: KLF5_HUMAN

NCBI Description

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane

Molecular Function: protein binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of microvillus biogenesis