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Typical Testing Data/Standard Curve (for reference only)

Human Keratan Sulfate (KS) ELISA Kit | KS elisa kit

Human Keratan Sulfate (KS) ELISA Kit

Gene Names
KERA; KTN; CNA2; SLRR2B
Reactivity
Human
Synonyms
Keratan Sulfate (KS); Human Keratan Sulfate (KS) ELISA Kit; Keratosulfate; KS elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Keratan Sulfate (KS).
No significant cross-reactivity or interference between Keratan Sulfate (KS) and analogues was observed.
Sequence Length
352
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
39.06-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 14.94pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratan Sulfate (KS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratan Sulfate (KS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for KS elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Keratan Sulfate (KS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Keratan Sulfate (KS) and unlabeled Keratan Sulfate (KS) (Standards or samples) with the pre-coated antibody specific to Keratan Sulfate (KS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Keratan Sulfate (KS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Keratan Sulfate (KS) in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratocan
NCBI Official Synonym Full Names
keratocan
NCBI Official Symbol
KERA
NCBI Official Synonym Symbols
KTN; CNA2; SLRR2B
NCBI Protein Information
keratocan
UniProt Protein Name
Keratocan
UniProt Gene Name
KERA
UniProt Synonym Gene Names
SLRR2B; KTN
UniProt Entry Name
KERA_HUMAN

NCBI Description

The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2).[provided by RefSeq, May 2010]

Uniprot Description

KERA: May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix. Defects in KERA are the cause of the autosomal recessive cornea plana 2 (CNA2). In CNA2, the forward convex curvature is flattened, leading to a decrease in refraction, reduced visual activity, extreme hyperopia (usually plus 10 d or more), hazy corneal limbus, opacities in the corneal parenchyma, and marked arcus senilis (often detected at an early age). CNA2 is a rare disorder with a worldwide distribution, but a high prevalence in the Finnish population. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular region; extracellular space; Golgi lumen; lysosomal lumen; proteinaceous extracellular matrix

Biological Process: axonogenesis; keratan sulfate biosynthetic process; keratan sulfate catabolic process; response to stimulus; visual perception

Disease: Cornea Plana 2

Research Articles on KS

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Product Notes

The Human KS kera (Catalog #AAA2707387) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707387 ELISA Kit recognizes Human KS. It is sometimes possible for the material contained within the vial of "Keratan Sulfate (KS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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