Human Kallikrein 11 (KLK11) ELISA Kit | KLK11 elisa kit

Human Kallikrein 11 (KLK11) ELISA Kit

Cat. Num. AAA2021338

• Gene Names: KLK11; TLSP; PRSS20
• Reactivity: Human
• Synonyms: Kallikrein 11 (KLK11); Human Kallikrein 11 (KLK11) ELISA Kit; TLSP; hK11; Hippostatin; Kallikrein-Related Peptidase 11; Serine protease 20; Trypsin-like protease; KLK11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kallikrein 11 (KLK11).; No significant cross-reactivity or interference between Kallikrein 11 (KLK11) and analogues was observed.
• Sequence Length: 275

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 11 (KLK11) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 11 (KLK11) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK11 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 11 (KLK11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 11 (KLK11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 11 (KLK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 11 (KLK11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KLK11 elisa kit

Enzyme & Kinase; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 262527289
• NCBI GeneID: 11012
• NCBI Accession #: NP_001161077.1
• NCBI GenBank Nucleotide #: NM_001167605.1
• UniProt Accession #: Q9UBX7; O75837; Q0WXX5; Q8IXD7; Q9NS65
• Molecular Weight: 30,166 Da
• NCBI Official Full Name: kallikrein-11 isoform 3
• NCBI Official Synonym Full Names: kallikrein-related peptidase 11
• NCBI Official Symbol: KLK11
• NCBI Official Synonym Symbols: TLSP; PRSS20
• NCBI Protein Information: kallikrein-11
• UniProt Protein Name: Kallikrein-11
• Protein Family: Kallikrein
• UniProt Gene Name: KLK11
• UniProt Synonym Gene Names: PRSS20; TLSP; hK11
• UniProt Entry Name: KLK11_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009]

Uniprot Description

KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: Golgi apparatus; extracellular space

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis