Human JTV1 (Protein JTV-1) ELISA Kit | JTV1 elisa kit

Human JTV1 (Protein JTV-1)ELISA Kit

Cat. Num. AAA7608227

• Gene Names: AIMP2; P38; JTV1; JTV-1; PRO0992
• Reactivity: Human
• Synonyms: JTV1 (Protein JTV-1); Human JTV1 (Protein JTV-1)ELISA Kit; AIMP2; JTV1; JTV1 gene; P38; PRO0992; Protein JTV 1; JTV1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of JTV1. No significant cross-reactivity or interference between JTV1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JTV1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11125770
• NCBI GeneID: 7965
• NCBI Accession #: NP_006294
• NCBI GenBank Nucleotide #: NM_006303.3
• UniProt Accession #: Q13155; Q75MR1; Q96CZ5; Q9P1L2
• Molecular Weight: Predicted: 35 kDa; ; Observed: 36 kDa
• NCBI Official Full Name: aminoacyl tRNA synthase complex-interacting multifunctional protein 2
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
• NCBI Official Symbol: AIMP2
• NCBI Official Synonym Symbols: P38; JTV1; JTV-1; PRO0992
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 2; protein JTV-1; ARS-interacting multi-functional protein 2; multisynthase complex auxiliary component p38; multisynthetase complex auxiliary component p38
• UniProt Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 2
• UniProt Gene Name: AIMP2
• UniProt Synonym Gene Names: JTV1
• UniProt Entry Name: AIMP2_HUMAN

NCBI Description

The JTV1 gene is located on chromosome 7p22 flanked by two genes, HRI and PMS2. JTV1 and HRI overlap slightly and are arranged in a tail-to-tail fashion. JTV1 and PMS2 are separated by approximately 200 base pairs and are arranged head-to-head. JTV1 is transcribed in the opposite direction compared to HRI and PMS2. The function of the JTV1 gene product is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

JTV1: Required for assembly and stability of the aminoacyl- tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down- regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: membrane; nucleus; cytosol

Molecular Function: protein binding

Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; positive regulation of protein ubiquitination; apoptosis; gene expression