Parathyroid hormone ELISA Kit | Pth elisa kit

Rat Parathyroid hormone ELISA Kit

Cat. Num. AAA764729

• Gene Names: PTH; PTH1
• Reactivity: Rat
• Synonyms: Parathyroid hormone; Rat Parathyroid hormone ELISA Kit; Pth (Parathyroid hormone)/Parathormone/Parathyrin/Parathormone/parathormone/Parathyrin/parathyrin/parathyroid hormone/parathyroid hormone 1/PTH1; Pth elisa kit

• Reactivity: Rat
• Sequence Length: 115

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800pg/ml
• Sensitivity: 7.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Pth elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat Pth antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Pth antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Pth amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Pth can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506267
• NCBI GeneID: 5741
• NCBI Accession #: NP_000306.1
• NCBI GenBank Nucleotide #: NM_000315.3
• Molecular Weight: 12,861 Da
• NCBI Official Full Name: parathyroid hormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: parathyroid hormone
• NCBI Official Symbol: PTH
• NCBI Official Synonym Symbols: PTH1
• NCBI Protein Information: parathyroid hormone
• UniProt Protein Name: Parathyroid hormone
• Protein Family: Parathyroid hormone
• UniProt Gene Name: PTH
• UniProt Synonym Gene Names: PTH
• UniProt Entry Name: PTHY_HUMAN

NCBI Description

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PTH: PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2- deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Belongs to the parathyroid hormone family.

Protein type: Secreted; Secreted, signal peptide; Apoptosis; Hormone

Chromosomal Location of Human Ortholog: 11p15.3-p15.1

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: hormone activity; parathyroid hormone receptor binding; peptide hormone receptor binding; RNA polymerase II transcription factor activity, enhancer binding; type 1 parathyroid hormone receptor binding

Biological Process: bone resorption; cAMP metabolic process; cell-cell signaling; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; induction of apoptosis by hormones; negative regulation of transcription from RNA polymerase II promoter; positive regulation of bone mineralization; positive regulation of cAMP biosynthetic process; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter; regulation of gene expression; response to cadmium ion; response to drug; response to ethanol; response to lead ion; response to vitamin D; Rho protein signal transduction; skeletal development

Disease: Hypoparathyroidism, Familial Isolated