Human interferon regulatory factor 8, IRF8 ELISA Kit | IRF8 elisa kit

Human interferon regulatory factor 8, IRF8 ELISA Kit

Cat. Num. AAA163525

• Gene Names: IRF8; ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• Reactivity: Human
• Synonyms: interferon regulatory factor 8; IRF8; Human interferon regulatory factor 8; IRF8 ELISA Kit; IRF8 elisa kit

• Reactivity: Human
• Sequence Length: 426

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 5ng/L - 1000ng/L
• Sensitivity: 2.44ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF8 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Interferon Regulatory Factor 8 (also known as IRF8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human IRF8 antibody. IRF8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human IRF8 Antibody is added and binds to IRF8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IRF8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IRF8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4504567
• NCBI GeneID: 3394
• NCBI Accession #: NP_002154.1
• NCBI GenBank Nucleotide #: NM_002163.3
• UniProt Accession #: Q02556; A0AV82
• Molecular Weight: 48,356 Da
• NCBI Official Full Name: interferon regulatory factor 8 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 8
• NCBI Official Symbol: IRF8
• NCBI Official Synonym Symbols: ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP
• NCBI Protein Information: interferon regulatory factor 8
• UniProt Protein Name: Interferon regulatory factor 8
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF8
• UniProt Synonym Gene Names: ICSBP1; IRF-8; H-ICSBP; ICSBP

NCBI Description

Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role as a transcriptional activator or repressor (PubMed:25122610). Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes ().