Typical Testing Data/Standard Curve (for reference only)
Human Integrin Beta 1 (ITGb1) ELISA Kit | ITGb1 elisa kit
Human Integrin Beta 1 (ITGb1) Mini Samples ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ITGb1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ITGb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).
Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 10p11.22
Cellular Component: cell surface; cell-cell adherens junction; cytoplasm; filopodium; focal adhesion; lipid raft; membrane; neuromuscular junction; plasma membrane; receptor complex; ruffle; sarcolemma
Molecular Function: actin binding; C-X3-C chemokine binding; cell adhesion molecule binding; coreceptor activity; fibronectin binding; protease binding; protein binding; protein complex binding; protein heterodimerization activity
Biological Process: B cell differentiation; calcium-independent cell-matrix adhesion; cell adhesion mediated by integrin; cell migration; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular defense response; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; homophilic cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; mesodermal cell differentiation; positive regulation of apoptosis; positive regulation of GTPase activity; receptor internalization; regulation of immune response; stress fiber formation; transforming growth factor beta receptor signaling pathway
Research Articles on ITGb1
Similar Products
Product Notes
The Human ITGb1 itgb1 (Catalog #AAA2087755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2087755 ELISA Kit recognizes Human ITGb1. It is sometimes possible for the material contained within the vial of "Integrin Beta 1 (ITGb1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
