Human Integrin beta-1 ELISA Kit | ITGB1 elisa kit

Human Integrin beta-1 ELISA Kit

Cat. Num. AAA2886562

• Reactivity: Human
• Synonyms: Integrin beta-1; Human Integrin beta-1 ELISA Kit; Fibronectin receptor subunit beta; VLA-4 subunit beta; ITGB1; FNRB; MDF2; MSK12; GPIIA; CD29; ITGB1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Aspartoacylase Synonyms: Aspartoacylase,
• Sequence Length: 798

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 78-5000 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=5.5%
• Inter-assay Precision: Inter-Assay CV: <=8.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGB1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19743813
• NCBI GeneID: 3688
• NCBI Accession #: NP_002202.2
• NCBI GenBank Nucleotide #: NM_002211.3
• UniProt Accession #: P05556; P78466; P78467; Q13089; Q13090; Q13091; Q13212; A8K6N2; D3DRX9; D3DRY3; D3DRY4; D3DRY5
• Molecular Weight: 88,884 Da
• NCBI Official Full Name: integrin beta-1 isoform 1A
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA
• UniProt Entry Name: ITB1_HUMAN

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: acrosome; basement membrane; cell surface; cleavage furrow; cytoplasm; dendritic spine; external side of plasma membrane; filopodium; focal adhesion; hemidesmosome; integrin complex; lamellipodium; lipid raft; melanosome; membrane; neuromuscular junction; perinuclear region of cytoplasm; plasma membrane; receptor complex; recycling endosome; ruffle; sarcolemma

Molecular Function: actin binding; alpha-actinin binding; cell adhesion molecule binding; fibronectin binding; glycoprotein binding; integrin binding; laminin binding; metal ion binding; peptide binding; protease binding; protein binding; protein complex binding; protein domain specific binding; protein heterodimerization activity; protein kinase binding; viral receptor activity

Biological Process: axon extension; axon guidance; B cell differentiation; blood coagulation; calcium-independent cell-matrix adhesion; cardiac muscle cell differentiation; cell fate specification; cell migration; cell migration during sprouting angiogenesis; cell-cell adhesion mediated by integrin; cell-matrix adhesion; cell-substrate adhesion; cellular calcium ion homeostasis; cellular defense response; dendrite morphogenesis; entry of virus into host cell; extracellular matrix organization and biogenesis; formation of radial glial scaffolds; G1/S transition of mitotic cell cycle; germ cell migration; heterotypic cell-cell adhesion; homophilic cell adhesion; in utero embryonic development; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; maternal process involved in pregnancy; mesodermal cell differentiation; negative regulation of cell projection organization and biogenesis; negative regulation of cell proliferation; negative regulation of neuron differentiation; negative regulation of Rho protein signal transduction; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endocytosis; positive regulation of GTPase activity; positive regulation of MAPKKK cascade; positive regulation of peptidyl-tyrosine phosphorylation; protein transport within lipid bilayer; receptor internalization; regulation of cell cycle; regulation of G-protein coupled receptor protein signaling pathway; regulation of immune response; response to activity; response to drug; sarcomere organization; small GTPase mediated signal transduction; stress fiber formation; tissue homeostasis; transforming growth factor beta receptor signaling pathway; visual learning