Human Integrator complex subunit 10 (INTS10) ELISA Kit | INTS10 elisa kit

Human Integrator complex subunit 10 (INTS10) ELISA Kit

Cat. Num. AAA2802176

• Gene Names: INTS10; INT10; C8orf35
• Reactivity: Human
• Synonyms: Integrator complex subunit 10 (INTS10); Human Integrator complex subunit 10 (INTS10) ELISA Kit; C8orf35; FLJ10569; INT10; INTS10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human INTS10. No significant cross-reactivity or interference between Human INTS10 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate INTS10 in samples. An antibody specific for INTS10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyINTS10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INTS10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INTS10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 83582809
• NCBI GeneID: 55174
• NCBI Accession #: NP_060612.2
• NCBI GenBank Nucleotide #: NM_018142.2
• UniProt Accession #: Q9NVR2; Q6IA93; Q7L538; Q7L8C8; Q9H3W8
• Molecular Weight: 82,236 Da
• NCBI Official Full Name: integrator complex subunit 10
• NCBI Official Synonym Full Names: integrator complex subunit 10
• NCBI Official Symbol: INTS10
• NCBI Official Synonym Symbols: INT10; C8orf35
• NCBI Protein Information: integrator complex subunit 10
• UniProt Protein Name: Integrator complex subunit 10
• Protein Family: Integrator complex
• UniProt Gene Name: INTS10
• UniProt Synonym Gene Names: C8orf35; Int10
• UniProt Entry Name: INT10_HUMAN

NCBI Description

INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]

Uniprot Description

INTS10: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Belongs to the Integrator subunit 10 family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: integrator complex

Molecular Function: protein binding

Biological Process: snRNA processing