Typical Testing Data/Standard Curve (for reference only)
Human Insulin Autoantibody (IAA) ELISA Kit | IAA elisa kit
Human Insulin Autoantibody (IAA) ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Insulin Autoantibody (IAA); Human Insulin Autoantibody (IAA) ELISA Kit; Insulin Autoantibody; Anti-Insulin Antibody; Antiinsulin; Insulin Antibodies; IAA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Insulin Autoantibody (IAA).
No significant cross-reactivity or interference between Insulin Autoantibody (IAA) and analogues was observed.
No significant cross-reactivity or interference between Insulin Autoantibody (IAA) and analogues was observed.
Applicable Applications for IAA elisa kit
ELISA (EIA)
Assay Type
Competitive Inhibition
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.12ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Insulin Autoantibody (IAA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Autoantibody (IAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
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Product Notes
The Human IAA (Catalog #AAA2707865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2707865 ELISA Kit recognizes Human IAA. AAA Biotech's Insulin Autoantibody (IAA) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the IAA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Autoantibody (IAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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